This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mosammaparast, N.
Right arrow Articles by Pemberton, L. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mosammaparast, N.
Right arrow Articles by Pemberton, L. F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2005, p. 1764-1778, Vol. 25, No. 5
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.5.1764-1778.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Modulation of Histone Deposition by the Karyopherin Kap114

Nima Mosammaparast, Brian C. Del Rosario, and Lucy F. Pemberton*

Center for Cell Signaling and Department of Microbiology, University of Virginia, Charlottesville, Virginia

Received 21 June 2004/ Returned for modification 26 July 2004/ Accepted 29 November 2004

The nuclear import of histones is a prerequisite for the downstream deposition of histones to form chromatin. However, the coordinate regulation of these processes remains poorly understood. Here we demonstrate that Kap114p, the primary karyopherin/importin responsible for the nuclear import of histones H2A and H2B, modulates the deposition of histones H2A and H2B by the histone chaperone Nap1p. We show that a complex comprising Kap114p, histones H2A and H2B, and Nap1p is present in the nucleus and that the presence of this complex is specifically promoted by Nap1p. This places Kap114p in a position to modulate Nap1p function, and we demonstrate by the use of two different assay systems that Kap114p inhibits Nap1p-mediated chromatin assembly. The inhibition of H2A and H2B deposition by Kap114p results in the concomitant inhibition of RCC1 loading onto chromatin. Biochemical evidence suggests that the mechanism by which Kap114p modulates histone deposition primarily involves direct histone binding, while the interaction between Kap114p and Nap1p plays a secondary role. Furthermore, we found that the inhibition of histone deposition by Kap114p is partially reversed by RanGTP. Our results indicate a novel mechanism by which cells can regulate histone deposition and establish a coordinate link between histone nuclear import and chromatin assembly.

* Corresponding author. Mailing address: Center for Cell Signaling, University of Virginia HSC, Box 800577, Charlottesville, VA 22908. Phone: (434) 243-6737. Fax: (434) 924-1236. E-mail: lfp2n{at}virginia.edu.

Molecular and Cellular Biology, March 2005, p. 1764-1778, Vol. 25, No. 5
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.5.1764-1778.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Milks, K. J., Moree, B., Straight, A. F. (2009). Dissection of CENP-C-directed Centromere and Kinetochore Assembly. Mol. Biol. Cell 20: 4246-4255 [Abstract] [Full Text]  
  • Del Rosario, B. C., Pemberton, L. F. (2008). Nap1 Links Transcription Elongation, Chromatin Assembly, and Messenger RNP Complex Biogenesis. Mol. Cell. Biol. 28: 2113-2124 [Abstract] [Full Text]  
  • Calvert, M. E. K., Keck, K. M., Ptak, C., Shabanowitz, J., Hunt, D. F., Pemberton, L. F. (2008). Phosphorylation by Casein Kinase 2 Regulates Nap1 Localization and Function. Mol. Cell. Biol. 28: 1313-1325 [Abstract] [Full Text]  
  • Zlatanova, J., Seebart, C., Tomschik, M. (2007). Nap1: taking a closer look at a juggler protein of extraordinary skills. FASEB J. 21: 1294-1310 [Abstract] [Full Text]  
  • Park, Y.-J., Luger, K. (2006). The structure of nucleosome assembly protein 1. Proc. Natl. Acad. Sci. USA 103: 1248-1253 [Abstract] [Full Text]  
  • Hodges, J. L., Leslie, J. H., Mosammaparast, N., Guo, Y., Shabanowitz, J., Hunt, D. F., Pemberton, L. F. (2005). Nuclear Import of TFIIB Is Mediated by Kap114p, a Karyopherin with Multiple Cargo-binding Domains. Mol. Biol. Cell 16: 3200-3210 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»