This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prather, D. M.
Right arrow Articles by Winston, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prather, D. M.
Right arrow Articles by Winston, F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2005, p. 2650-2659, Vol. 25, No. 7
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.7.2650-2659.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evidence that the Elongation Factor TFIIS Plays a Role in Transcription Initiation at GAL1 in Saccharomyces cerevisiae

Donald M. Prather, Erica Larschan, and Fred Winston*

Department of Genetics, Harvard Medical School, Boston, Massachusetts

Received 23 November 2004/ Returned for modification 21 December 2004/ Accepted 27 December 2004

TFIIS is a transcription elongation factor that has been extensively studied biochemically. Although the in vitro mechanisms by which TFIIS stimulates RNA transcript cleavage and polymerase read-through have been well characterized, its in vivo roles remain unclear. To better understand TFIIS function in vivo, we have examined its role during Gal4-mediated activation of the Saccharomyces cerevisiae GAL1 gene. Surprisingly, TFIIS is strongly associated with the GAL1 upstream activating sequence. In addition, TFIIS recruitment to Gal4-binding sites is dependent on Gal4, SAGA, and Mediator but not on RNA polymerase II (Pol II). The association of TFIIS is also necessary for the optimal recruitment of TATA-binding protein and Pol II to the GAL1 promoter. These results provide strong evidence that TFIIS plays an important role in the initiation of transcription at GAL1 in addition to its well-characterized roles in transcription elongation.

* Corresponding author. Mailing address: Department of Genetics, Harvard Medical School, 77 Louis Pasteur Ave., Boston, MA 02115. Phone: (617) 432-7768. Fax: (617) 432-6506. E-mail: winston{at}genetics.med.harvard.edu.

Molecular and Cellular Biology, April 2005, p. 2650-2659, Vol. 25, No. 7
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.7.2650-2659.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, Y., Warfield, L., Zhang, C., Luo, J., Allen, J., Lang, W. H., Ranish, J., Shokat, K. M., Hahn, S. (2009). Phosphorylation of the Transcription Elongation Factor Spt5 by Yeast Bur1 Kinase Stimulates Recruitment of the PAF Complex. Mol. Cell. Biol. 29: 4852-4863 [Abstract] [Full Text]  
  • Nagata, M., Ito, T., Arimitsu, N., Koyama, H., Sekimizu, K. (2009). Transcription arrest relief by S-II/TFIIS during gene expression in erythroblast differentiation. GENES CELLS 14: 371-380 [Abstract] [Full Text]  
  • Ghavi-Helm, Y., Michaut, M., Acker, J., Aude, J.-C., Thuriaux, P., Werner, M., Soutourina, J. (2008). Genome-wide location analysis reveals a role of TFIIS in RNA polymerase III transcription. Genes Dev. 22: 1934-1947 [Abstract] [Full Text]  
  • Khaperskyy, D. A., Ammerman, M. L., Majovski, R. C., Ponticelli, A. S. (2008). Functions of Saccharomyces cerevisiae TFIIF during Transcription Start Site Utilization. Mol. Cell. Biol. 28: 3757-3766 [Abstract] [Full Text]  
  • Zhang, L., Fletcher, A. G. L., Cheung, V., Winston, F., Stargell, L. A. (2008). Spn1 Regulates the Recruitment of Spt6 and the Swi/Snf Complex during Transcriptional Activation by RNA Polymerase II. Mol. Cell. Biol. 28: 1393-1403 [Abstract] [Full Text]  
  • Guglielmi, B., Soutourina, J., Esnault, C., Werner, M. (2007). TFIIS elongation factor and Mediator act in conjunction during transcription initiation in vivo. Proc. Natl. Acad. Sci. USA 104: 16062-16067 [Abstract] [Full Text]  
  • Kim, B., Nesvizhskii, A. I., Rani, P. G., Hahn, S., Aebersold, R., Ranish, J. A. (2007). The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Proc. Natl. Acad. Sci. USA 104: 16068-16073 [Abstract] [Full Text]  
  • Biswas, D., Dutta-Biswas, R., Stillman, D. J. (2007). Chd1 and yFACT Act in Opposition in Regulating Transcription. Mol. Cell. Biol. 27: 6279-6287 [Abstract] [Full Text]  
  • Alic, N., Ayoub, N., Landrieux, E., Favry, E., Baudouin-Cornu, P., Riva, M., Carles, C. (2007). Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription. Proc. Natl. Acad. Sci. USA 104: 10400-10405 [Abstract] [Full Text]  
  • Fish, R. N., Ammerman, M. L., Davie, J. K., Lu, B. F., Pham, C., Howe, L., Ponticelli, A. S., Kane, C. M. (2006). Genetic Interactions Between TFIIF and TFIIS. Genetics 173: 1871-1884 [Abstract] [Full Text]  
  • Potrykus, K., Vinella, D., Murphy, H., Szalewska-Palasz, A., D'Ari, R., Cashel, M. (2006). Antagonistic Regulation of Escherichia coli Ribosomal RNA rrnB P1 Promoter Activity by GreA and DksA. J. Biol. Chem. 281: 15238-15248 [Abstract] [Full Text]  
  • Ito, T., Arimitsu, N., Takeuchi, M., Kawamura, N., Nagata, M., Saso, K., Akimitsu, N., Hamamoto, H., Natori, S., Miyajima, A., Sekimizu, K. (2006). Transcription Elongation Factor S-II Is Required for Definitive Hematopoiesis. Mol. Cell. Biol. 26: 3194-3203 [Abstract] [Full Text]  
  • Malagon, F., Kireeva, M. L., Shafer, B. K., Lubkowska, L., Kashlev, M., Strathern, J. N. (2006). Mutations in the Saccharomyces cerevisiae RPB1 Gene Conferring Hypersensitivity to 6-Azauracil. Genetics 172: 2201-2209 [Abstract] [Full Text]  
  • Prather, D., Krogan, N. J., Emili, A., Greenblatt, J. F., Winston, F. (2005). Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 10122-10135 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»