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Molecular and Cellular Biology, April 2005, p. 2969-2980, Vol. 25, No. 8
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.8.2969-2980.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing

Demian Cazalla, Kathryn Newton,{dagger} and Javier F. Cáceres*

MRC Human Genetics Unit, Edinburgh, Scotland, United Kingdom

Received 15 November 2004/ Returned for modification 15 December 2004/ Accepted 10 January 2005

The SR family proteins and SR-related polypeptides are important regulators of pre-mRNA splicing. A novel SR-related protein of an apparent molecular mass of 53 kDa was isolated in a gene trap screen that identifies proteins which localize to the nuclear speckles. This novel protein possesses an arginine- and serine-rich domain and was termed SRrp53 (for SR-related protein of 53 kDa). In support for a role of this novel RS-containing protein in pre-mRNA splicing, we identified the mouse ortholog of the Saccharomyces cerevisiae U1 snRNP-specific protein Luc7p and the U2AF65-related factor HCC1 as interacting proteins. In addition, SRrp53 is able to interact with some members of the SR family of proteins and with U2AF35 in a yeast two-hybrid system and in cell extracts. We show that in HeLa nuclear extracts immunodepleted of SRrp53, the second step of pre-mRNA splicing is blocked, and recombinant SRrp53 is able to restore splicing activity. SRrp53 also regulates alternative splicing in a concentration-dependent manner. Taken together, these results suggest that SRrp53 is a novel SR-related protein that has a role both in constitutive and in alternative splicing.

* Corresponding author. Mailing address: MRC Human Genetics Unit, Western General Hospital, Crewe Rd., Edinburgh EH4 2XU, Scotland, United Kingdom. Phone: 44 131 467 8426. Fax: 44 131 467 8456. E-mail: Javier.Caceres{at}hgu.mrc.ac.uk.

{dagger} Present address: Virginia Mason Research Center, Seattle, WA 98101.

Molecular and Cellular Biology, April 2005, p. 2969-2980, Vol. 25, No. 8
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.8.2969-2980.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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