This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuwahara, K.
Right arrow Articles by Olson, E. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuwahara, K.
Right arrow Articles by Olson, E. N.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2005, p. 3173-3181, Vol. 25, No. 8
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.8.3173-3181.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Muscle-Specific Signaling Mechanism That Links Actin Dynamics to Serum Response Factor{dagger}

Koichiro Kuwahara, Tomasa Barrientos, G. C. Teg Pipes, Shijie Li, and Eric N. Olson*

Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas

Received 28 September 2004/ Returned for modification 7 November 2004/ Accepted 19 January 2005

Myocardin and the myocardin-related transcription factors (MRTFs) MRTF-A and MRTF-B are coactivators for serum response factor (SRF), which regulates genes involved in cell proliferation, migration, cytoskeletal dynamics, and myogenesis. MRTF-A has been shown to translocate to the nucleus and activate SRF in response to Rho signaling and actin polymerization. Previously, we described a muscle-specific actin-binding protein named striated muscle activator of Rho signaling (STARS) that also activates SRF through a Rho-dependent mechanism. Here we show that STARS activates SRF by inducing the nuclear translocation of MRTFs. The STARS-dependent nuclear import of MRTFs requires RhoA and actin polymerization, and the actin-binding domain of STARS is necessary and sufficient for this activity. A knockdown of endogenous STARS expression by using small interfering RNA significantly reduced SRF activity in differentiated C2C12 skeletal muscle cells and cardiac myocytes. The ability of STARS to promote the nuclear localization of MRTFs and SRF-mediated transcription provides a potential muscle-specific mechanism for linking changes in actin dynamics and sarcomere structure with striated muscle gene expression.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390. Phone: (214) 648-1187. Fax: (214) 648-1196. E-mail: eric.olson{at}utsouthwestern.edu.

{dagger} We dedicate this paper to the memory of Akiko Arai.

Molecular and Cellular Biology, April 2005, p. 3173-3181, Vol. 25, No. 8
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.8.3173-3181.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Giger, J. M., Bodell, P. W., Zeng, M., Baldwin, K. M., Haddad, F. (2009). Rapid muscle atrophy response to unloading: pretranslational processes involving MHC and actin. J. Appl. Physiol. 107: 1204-1212 [Abstract] [Full Text]  
  • Xin, M., Small, E. M., Sutherland, L. B., Qi, X., McAnally, J., Plato, C. F., Richardson, J. A., Bassel-Duby, R., Olson, E. N. (2009). MicroRNAs miR-143 and miR-145 modulate cytoskeletal dynamics and responsiveness of smooth muscle cells to injury. Genes Dev. 23: 2166-2178 [Abstract] [Full Text]  
  • Ford-Speelman, D. L., Roche, J. A., Bowman, A. L., Bloch, R. J. (2009). The Rho-Guanine Nucleotide Exchange Factor Domain of Obscurin Activates RhoA Signaling in Skeletal Muscle. Mol. Biol. Cell 20: 3905-3917 [Abstract] [Full Text]  
  • Hanna, M., Liu, H., Amir, J., Sun, Y., Morris, S. W., Siddiqui, M. A. Q., Lau, L. F., Chaqour, B. (2009). Mechanical Regulation of the Proangiogenic Factor CCN1/CYR61 Gene Requires the Combined Activities of MRTF-A and CREB-binding Protein Histone Acetyltransferase. J. Biol. Chem. 284: 23125-23136 [Abstract] [Full Text]  
  • Makarenkova, H. P., Gonzalez, K. N., Kiosses, W. B., Meech, R. (2009). Barx2 Controls Myoblast Fusion and Promotes MyoD-mediated Activation of the Smooth Muscle{alpha}-Actin Gene. J. Biol. Chem. 284: 14866-14874 [Abstract] [Full Text]  
  • Lamon, S., Wallace, M. A., Leger, B., Russell, A. P. (2009). Regulation of STARS and its downstream targets suggest a novel pathway involved in human skeletal muscle hypertrophy and atrophy. J. Physiol. 587: 1795-1803 [Abstract] [Full Text]  
  • Stern, S., Debre, E., Stritt, C., Berger, J., Posern, G., Knoll, B. (2009). A Nuclear Actin Function Regulates Neuronal Motility by Serum Response Factor-Dependent Gene Transcription. J. Neurosci. 29: 4512-4518 [Abstract] [Full Text]  
  • Iwasaki, K., Hayashi, K., Fujioka, T., Sobue, K. (2008). Rho/Rho-associated Kinase Signal Regulates Myogenic Differentiation via Myocardin-related Transcription Factor-A/Smad-dependent Transcription of the Id3 Gene. J. Biol. Chem. 283: 21230-21241 [Abstract] [Full Text]  
  • Guettler, S., Vartiainen, M. K., Miralles, F., Larijani, B., Treisman, R. (2008). RPEL Motifs Link the Serum Response Factor Cofactor MAL but Not Myocardin to Rho Signaling via Actin Binding. Mol. Cell. Biol. 28: 732-742 [Abstract] [Full Text]  
  • Pelosi, M., Marampon, F., Zani, B. M., Prudente, S., Perlas, E., Caputo, V., Cianetti, L., Berno, V., Narumiya, S., Kang, S. W., Musaro, A., Rosenthal, N. (2007). ROCK2 and Its Alternatively Spliced Isoform ROCK2m Positively Control the Maturation of the Myogenic Program. Mol. Cell. Biol. 27: 6163-6176 [Abstract] [Full Text]  
  • L'honore, A., Rana, V., Arsic, N., Franckhauser, C., Lamb, N. J., Fernandez, A. (2007). Identification of a New Hybrid Serum Response Factor and Myocyte Enhancer Factor 2-binding Element in MyoD Enhancer Required for MyoD Expression during Myogenesis. Mol. Biol. Cell 18: 1992-2001 [Abstract] [Full Text]  
  • Dogra, C., Hall, S. L., Wedhas, N., Linkhart, T. A., Kumar, A. (2007). Fibroblast Growth Factor Inducible 14 (Fn14) Is Required for the Expression of Myogenic Regulatory Factors and Differentiation of Myoblasts into Myotubes: EVIDENCE FOR TWEAK-INDEPENDENT FUNCTIONS OF Fn14 DURING MYOGENESIS. J. Biol. Chem. 282: 15000-15010 [Abstract] [Full Text]  
  • Zhao, X.-H., Laschinger, C., Arora, P., Szaszi, K., Kapus, A., McCulloch, C. A. (2007). Force activates smooth muscle {alpha}-actin promoter activity through the Rho signaling pathway. J. Cell Sci. 120: 1801-1809 [Abstract] [Full Text]  
  • Ma, X., Renda, M. J., Wang, L., Cheng, E.-c., Niu, C., Morris, S. W., Chi, A. S., Krause, D. S. (2007). Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation. Mol. Cell. Biol. 27: 3056-3064 [Abstract] [Full Text]  
  • Barrientos, T., Frank, D., Kuwahara, K., Bezprozvannaya, S., Pipes, G. C. T., Bassel-Duby, R., Richardson, J. A., Katus, H. A., Olson, E. N., Frey, N. (2007). Two Novel Members of the ABLIM Protein Family, ABLIM-2 and -3, Associate with STARS and Directly Bind F-actin. J. Biol. Chem. 282: 8393-8403 [Abstract] [Full Text]  
  • Parmacek, M. S. (2007). Myocardin-Related Transcription Factors: Critical Coactivators Regulating Cardiovascular Development and Adaptation. Circ. Res. 100: 633-644 [Abstract] [Full Text]  
  • Staus, D. P., Blaker, A. L., Taylor, J. M., Mack, C. P. (2007). Diaphanous 1 and 2 Regulate Smooth Muscle Cell Differentiation by Activating the Myocardin-Related Transcription Factors. Arterioscler. Thromb. Vasc. Bio. 27: 478-486 [Abstract] [Full Text]  
  • Fan, L., Sebe, A., Peterfi, Z., Masszi, A., Thirone, A. C.P., Rotstein, O. D., Nakano, H., McCulloch, C. A., Szaszi, K., Mucsi, I., Kapus, A. (2007). Cell Contact-dependent Regulation of Epithelial-Myofibroblast Transition via the Rho-Rho Kinase-Phospho-Myosin Pathway. Mol. Biol. Cell 18: 1083-1097 [Abstract] [Full Text]  
  • Mebarek, S., Komati, H., Naro, F., Zeiller, C., Alvisi, M., Lagarde, M., Prigent, A.-F., Nemoz, G. (2007). Inhibition of de novo ceramide synthesis upregulates phospholipase D and enhances myogenic differentiation. J. Cell Sci. 120: 407-416 [Abstract] [Full Text]  
  • Miano, J. M., Long, X., Fujiwara, K. (2007). Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus. Am. J. Physiol. Cell Physiol. 292: C70-C81 [Abstract] [Full Text]  
  • Zeidan, A., Javadov, S., Karmazyn, M. (2006). Essential role of Rho/ROCK-dependent processes and actin dynamics in mediating leptin-induced hypertrophy in rat neonatal ventricular myocytes. Cardiovasc Res 72: 101-111 [Abstract] [Full Text]  
  • Charvet, C., Houbron, C., Parlakian, A., Giordani, J., Lahoute, C., Bertrand, A., Sotiropoulos, A., Renou, L., Schmitt, A., Melki, J., Li, Z., Daegelen, D., Tuil, D. (2006). New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways.. Mol. Cell. Biol. 26: 6664-6674 [Abstract] [Full Text]  
  • Li, S., Chang, S., Qi, X., Richardson, J. A., Olson, E. N. (2006). Requirement of a Myocardin-Related Transcription Factor for Development of Mammary Myoepithelial Cells. Mol. Cell. Biol. 26: 5797-5808 [Abstract] [Full Text]  
  • Pipes, G.C. T., Creemers, E. E., Olson, E. N. (2006). The myocardin family of transcriptional coactivators: versatile regulators of cell growth, migration, and myogenesis.. Genes Dev. 20: 1545-1556 [Abstract] [Full Text]  
  • Wamhoff, B. R., Bowles, D. K., Owens, G. K. (2006). Excitation-Transcription Coupling in Arterial Smooth Muscle. Circ. Res. 98: 868-878 [Abstract] [Full Text]  
  • Hoshijima, M. (2006). Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structures. Am. J. Physiol. Heart Circ. Physiol. 290: H1313-H1325 [Abstract] [Full Text]  
  • Oh, J., Richardson, J. A., Olson, E. N. (2005). Requirement of myocardin-related transcription factor-B for remodeling of branchial arch arteries and smooth muscle differentiation. Proc. Natl. Acad. Sci. USA 102: 15122-15127 [Abstract] [Full Text]  
  • Li, J., Zhu, X., Chen, M., Cheng, L., Zhou, D., Lu, M. M., Du, K., Epstein, J. A., Parmacek, M. S. (2005). Myocardin-related transcription factor B is required in cardiac neural crest for smooth muscle differentiation and cardiovascular development. Proc. Natl. Acad. Sci. USA 102: 8916-8921 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»