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Molecular and Cellular Biology, April 2005, p. 3317-3323, Vol. 25, No. 8
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.8.3317-3323.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

YB-1 Autoregulates Translation of Its Own mRNA at or prior to the Step of 40S Ribosomal Subunit Joining

Olga V. Skabkina,{dagger} Dmitry N. Lyabin,{dagger} Maxim A. Skabkin, and Lev P. Ovchinnikov*

Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia

Received 23 December 2004/ Accepted 16 January 2005

YB-1 is a member of the numerous families of proteins with an evolutionary ancient cold-shock domain. It is involved in many DNA- and RNA-dependent events and regulates gene expression at different levels. Previously, we found a regulatory element within the 3' untranslated region (UTR) of YB-1 mRNA that specifically interacted with YB-1 and poly(A)-binding protein (PABP); we also showed that PABP positively affected YB-1 mRNA translation in a poly(A) tail-independent manner (O. V. Skabkina, M. A. Skabkin, N. V. Popova, D. N. Lyabin, L. O. Penalva, and L. P. Ovchinnikov, J. Biol. Chem. 278:18191-18198, 2003). Here, YB-1 is shown to strongly and specifically inhibit its own synthesis at the stage of initiation, with accumulation of its mRNA in the form of free mRNPs. YB-1 and PABP binding sites have been mapped on the YB-1 mRNA regulatory element. These were UCCAG/ACAA for YB-1 and a ~50-nucleotide A-rich sequence for PABP that overlapped each other. PABP competes with YB-1 for binding to the YB-1 mRNA regulatory element and restores translational activity of YB-1 mRNA that has been inhibited by YB-1. Thus, YB-1 negatively regulates its own synthesis, presumably by specific interaction with the 3'UTR regulatory element, whereas PABP restores translational activity of YB-1 mRNA by displacing YB-1 from this element.

* Corresponding author. Mailing address: Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia. Phone and Fax: 7-095-632-78-71. E-mail: ovchinn{at}vega.protres.ru.

{dagger} O.V.S. and D.N.L. contributed equally to this work.

Molecular and Cellular Biology, April 2005, p. 3317-3323, Vol. 25, No. 8
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.8.3317-3323.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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