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Molecular and Cellular Biology, May 2005, p. 3763-3773, Vol. 25, No. 9
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.9.3763-3773.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction of Paxillin with Poly(A)-Binding Protein 1 and Its Role in Focal Adhesion Turnover and Cell Migration

Alison J. Woods,1 Theodoros Kantidakis,1,{dagger} Hisataka Sabe,2 David R. Critchley,1 and Jim C. Norman1*

Department of Biochemistry, University of Leicester, Leicester, United Kingdom,1 Department of Molecular Biology, Osaka Bioscience Institute, Suita, Osaka, Japan2

Received 24 May 2004/ Returned for modification 29 July 2004/ Accepted 10 January 2005

We have previously identified poly(A)-binding protein 1 (PABP1) as a ligand for paxillin and shown that the paxillin-PABP1 complex undergoes nucleocytoplasmic shuttling. By targeting the paxillin-binding subdomain sequences in PABP1, we have generated mutants of PABP1 that do not bind to cellular paxillin. Here we report that paxillin association is necessary for efficient nuclear export of PABP1 and that RNA interference of paxillin drives the nuclear accumulation of PABP1. Furthermore, ablation of paxillin-PABP1 association impeded a number of indices of cell motility including spreading on fibronectin, cell migration on two-dimensional matrices, and transmigration in Boyden chambers. These data indicate that PABP1 must associate with paxillin in order to be efficiently transported from the nucleus to the cytoplasm and that this event is necessary for cells to remodel their focal adhesions during cell migration.

* Corresponding author. Mailing address: Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7 RH, United Kingdom. Phone: 0116-252-5250. Fax: 0116-252-3369. E-mail: jcn2{at}le.ac.uk.

{dagger} Present address: Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Molecular and Cellular Biology, May 2005, p. 3763-3773, Vol. 25, No. 9
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.9.3763-3773.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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