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Molecular and Cellular Biology, January 2006, p. 100-116, Vol. 26, No. 1
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.1.100-116.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Distinct Utilization of Effectors and Biological Outcomes Resulting from Site-Specific Ras Activation: Ras Functions in Lipid Rafts and Golgi Complex Are Dispensable for Proliferation and Transformation

David Matallanas,^1, Victoria Sanz-Moreno,^1, Imanol Arozarena,¹ Fernando Calvo,¹ Lorena Agudo-Ibáñez,¹ Eugenio Santos,² María T. Berciano,³ and Piero Crespo^1*

Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas (CSIC), Departamento de Biología Molecular,¹ Departamento de Anatomía y Biología Celular, Unidad de Biomedicina, CSIC-Universidad de Cantabria, Santander 39011, Spain,³ Centro de Investigación del Cancer, IBMCC, CSIC-Universidad de Salamanca, Salamanca 37007, Spain²

Received 4 May 2005/ Returned for modification 2 June 2005/ Accepted 6 October 2005

Ras proteins are distributed in different types of plasma membrane microdomains and endomembranes. However, how microlocalization affects the signals generated by Ras and its subsequent biological outputs is largely unknown. We have approached this question by selectively targeting RasV12 to different cellular sublocalizations. We show here that compartmentalization dictates Ras utilization of effectors and the intensity of its signals. Activated Ras can evoke enhanced proliferation and transformation from most of its platforms, with the exception of the Golgi complex. Furthermore, signals that promote survival emanate primarily from the endoplasmic reticulum pool. In addition, we have investigated the need for the different pools of endogenous Ras in the conveyance of upstream mitogenic and transforming signals. Using targeted RasN17 inhibitory mutants and in physiological contexts such as H-Ras/N-Ras double knockout fibroblasts, we demonstrate that Ras functions at lipid rafts and at the Golgi complex are fully dispensable for proliferation and transformation.

D.M. and V.S.-M. contributed equally to this study.

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