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Molecular and Cellular Biology, January 2006, p. 293-302, Vol. 26, No. 1
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.1.293-302.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Innate Immune Responses in NF-B-Repressing Factor-Deficient Mice

Natali Froese,¹ Michael Schwarzer,^1, Ina Niedick,¹ Ursula Frischmann,² Mario Köster,¹ Andrea Kröger,¹ Peter P. Mueller,¹ Mahtab Nourbakhsh,^1, Bastian Pasche,³ Jörg Reimann,⁴ Peter Staeheli,⁵ and Hansjörg Hauser^1*

Department of Gene Regulation and Differentiation,¹ Department of Experimental Immunology,² Department of Infection Genetics, German Research Centre for Biotechnology, Braunschweig, Germany,³ Department of Internal Medicine I, University of Ulm, Ulm, Germany,⁴ Department of Virology, University of Freiburg, Freiburg, Germany⁵

Received 27 June 2005/ Returned for modification 7 August 2005/ Accepted 3 September 2005

NF-B-repressing factor (NRF) is a transcriptional silencer protein that specifically counteracts the basal activity of several NF-B-dependent promoters by direct binding to specific neighboring DNA sequences. In cell culture experiments, the reduction of NRF mRNA leads to a derepression of beta interferon, interleukin-8, and inducible nitric oxide synthase transcription. The X chromosome-located single-copy NRF gene is ubiquitously expressed and encodes a protein of 690 amino acids. The N-terminal part contains a nuclear localization signal, the DNA-binding domain, and the NF-B-repressing domain, while the C-terminal part is responsible for double-stranded RNA binding and nucleolar localization. To study the function of NRF in a systemic context, transgenic mice lacking the NRF gene were created. Against predictions from in vitro experiments, mice with a deletion of the NRF gene are viable and have a phenotype that is indistinguishable from wild-type mice, even after challenge with different pathogens. The data hint towards an unexpected functional redundancy of NRF.

Present address: Tulane University School of Medicine, Department of Medicine, New Orleans, La.

Present address: Institute of Pharmacology, Medical University of Hannover, Hannover, Germany.

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