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Molecular and Cellular Biology, June 2006, p. 3999-4005, Vol. 26, No. 11
0270-7306/06/$08.00+0     doi:10.1128/MCB.00293-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Requirement of ELC1 for RNA Polymerase II Polyubiquitylation and Degradation in Response to DNA Damage in Saccharomyces cerevisiae

Balazs Ribar, Louise Prakash, and Satya Prakash*

Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas

Received 16 February 2006/ Returned for modification 8 March 2006/ Accepted 9 March 2006

Treatment of Saccharomyces cerevisiae and human cells with DNA-damaging agents such as UV light or 4-nitroquinoline-1-oxide induces polyubiquitylation of the largest RNA polymerase II (Pol II) subunit, Rpb1, which results in rapid Pol II degradation by the proteasome. Here we identify a novel role for the yeast Elc1 protein in mediating Pol II polyubiquitylation and degradation in DNA-damaged yeast cells and propose the involvement of a ubiquitin ligase, of which Elc1 is a component, in this process. In addition, we present genetic evidence for a possible involvement of Elc1 in Rad7-Rad16-dependent nucleotide excision repair (NER) of lesions from the nontranscribed regions of the genome and suggest a role for Elc1 in increasing the proficiency of repair of nontranscribed DNA, where as a component of the Rad7-Rad16-Elc1 ubiquitin ligase, it would promote the efficient turnover of the NER ensemble from the lesion site in a Rad23-19S proteasomal complex-dependent reaction.

* Corresponding author. Mailing address: Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Blocker Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061. Phone: (409) 747-8602. Fax: (409) 747-8608. E-mail: s.prakash{at}utmb.edu.

Molecular and Cellular Biology, June 2006, p. 3999-4005, Vol. 26, No. 11
0270-7306/06/$08.00+0     doi:10.1128/MCB.00293-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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