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Molecular and Cellular Biology, July 2006, p. 4806-4817, Vol. 26, No. 13
0270-7306/06/$08.00+0     doi:10.1128/MCB.02062-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Interaction between PCNA and Diubiquitinated Mcm10 Is Essential for Cell Growth in Budding Yeast

Sapna Das-Bradoo, Robin M. Ricke, and Anja-Katrin Bielinsky*

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455

Received 24 October 2005/ Returned for modification 20 November 2005/ Accepted 19 April 2006

The minichromosome maintenance protein 10 (Mcm10) is an evolutionarily conserved factor that is essential for replication initiation and elongation. Mcm10 is part of the eukaryotic replication fork and interacts with a variety of proteins, including the Mcm2-7 helicase and DNA polymerase alpha/primase complexes. A motif search revealed a match to the proliferating cell nuclear antigen (PCNA)-interacting protein (PIP) box in Mcm10. Here, we demonstrate a direct interaction between Mcm10 and PCNA that is alleviated by mutations in conserved residues of the PIP box. Interestingly, only the diubiquitinated form of Mcm10 binds to PCNA. Diubiquitination of Mcm10 is cell cycle regulated; it first appears in late G1 and persists throughout S phase. During this time, diubiquitinated Mcm10 is associated with chromatin, suggesting a direct role in DNA replication. Surprisingly, a Y245A substitution in the PIP box of Mcm10 that inhibits the interaction with PCNA abolishes cell proliferation. This severe-growth phenotype, which has not been observed for analogous mutations in other PCNA-interacting proteins, is rescued by a compensatory mutation in PCNA that restores interaction with Mcm10-Y245A. Taken together, our results suggest that diubiquitinated Mcm10 interacts with PCNA to facilitate an essential step in DNA elongation.

* Corresponding author. Mailing address: University of Minnesota, Biochemistry, Molecular Biology and Biophysics, 321 Church Street SE, 6-155 Jackson Hall, Minneapolis, MN 55455. Phone: (612) 624-2469. Fax: (612) 625-2163. E-mail: bieli003{at}umn.edu.

Molecular and Cellular Biology, July 2006, p. 4806-4817, Vol. 26, No. 13
0270-7306/06/$08.00+0     doi:10.1128/MCB.02062-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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