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Molecular and Cellular Biology, July 2006, p. 5325-5335, Vol. 26, No. 14
0270-7306/06/$08.00+0     doi:10.1128/MCB.00584-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analysis of Human Histone H2AZ Deposition In Vivo Argues against Its Direct Role in Epigenetic Templating Mechanisms

Antoine Viens,1,{dagger} Undine Mechold,1,{dagger},{ddagger} Franck Brouillard,3 Cristele Gilbert,1 Philippe Leclerc,2 and Vasily Ogryzko1*

Oncogenese, Differenciation et Transduction du Signal, CNRS UPR 9079, Institut Andre Lwoff, Batiment B, 1er Etage, 7 rue Guy Moquet, 94800 Villejuif, France,1 ,2 Proteomic Core Facilities, Faculté demédecine René Descartes, 156, rue de Vaugirard, Paris F-75015, France,3 Service de Microscopie Confocale, INSERM IFR93, 80, rue du Général Leclerc, F-94276 Bicêtre CEDEX, France4

Received 4 April 2006/ Accepted 27 April 2006

Chromatin is considered to be a principal carrier of epigenetic information due to the ability of alternative chromatin states to persist through generations of cell divisions and to spread on DNA. Replacement histone variants are novel candidates for epigenetic marking of chromatin. We developed a novel approach to analyze the chromatin environment of nucleosomes containing a particular replacement histone. We applied it to human H2AZ, one of the most studied alternative histones. We find that neither H2AZ itself nor other features of the H2AZ-containing nucleosome spread to the neighboring nucleosomes in vivo, arguing against a role for H2AZ as a self-perpetuating epigenetic mark.

* Corresponding author. Present address: CNRS UMR 8126, Institut Gustave-Roussy, PR1, 39 rue Camille Desmoulin, 94100 Villejuif, France. Phone and fax: 33-1 42 11 65 25. E-mail: ogryzko{at}igr.fr.

{dagger} These authors contributed equally to this work.

{ddagger} Present address: Génétique des Génomes Bactériens, Genetics of Bacterial Genomes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.

Molecular and Cellular Biology, July 2006, p. 5325-5335, Vol. 26, No. 14
0270-7306/06/$08.00+0     doi:10.1128/MCB.00584-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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