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Molecular and Cellular Biology, July 2006, p. 5509-5517, Vol. 26, No. 14
0270-7306/06/$08.00+0     doi:10.1128/MCB.00307-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Functional and Physical Interaction of Yeast Mgs1 with PCNA: Impact on RAD6-Dependent DNA Damage Tolerance

Takashi Hishida,1* Tomoko Ohya,1 Yoshino Kubota,1 Yusuke Kamada,1 and Hideo Shinagawa1,2

Genome Dynamics Group, Research Institute for Microbial Diseases, Osaka University, Osaka 565-871, Japan,1 BioAcademia, Inc., Osaka 565-0085, Japan2

Received 18 February 2006/ Returned for modification 17 March 2006/ Accepted 26 April 2006

Proliferating cell nuclear antigen (PCNA), a sliding clamp required for processive DNA synthesis, provides attachment sites for various other proteins that function in DNA replication, DNA repair, cell cycle progression and chromatin assembly. It has been shown that differential posttranslational modifications of PCNA by ubiquitin or SUMO play a pivotal role in controlling the choice of pathway for rescuing stalled replication forks. Here, we explored the roles of Mgs1 and PCNA in replication fork rescue. We provide evidence that Mgs1 physically associates with PCNA and that Mgs1 helps suppress the RAD6 DNA damage tolerance pathway in the absence of exogenous DNA damage. We also show that PCNA sumoylation inhibits the growth of mgs1 rad18 double mutants, in which PCNA sumoylation and the Srs2 DNA helicase coordinately prevent RAD52-dependent homologous recombination. The proposed roles for Mgs1, Srs2, and modified PCNA during replication arrest highlight the importance of modulating the RAD6 and RAD52 pathways to avoid genome instability.

* Corresponding author. Mailing address: Genome Dynamics Group, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8319. Fax: 81-6-6879-8320. E-mail: hishida{at}biken.osaka-u.ac.jp.

Molecular and Cellular Biology, July 2006, p. 5509-5517, Vol. 26, No. 14
0270-7306/06/$08.00+0     doi:10.1128/MCB.00307-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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