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Molecular and Cellular Biology, August 2006, p. 6239-6247, Vol. 26, No. 16
0270-7306/06/$08.00+0     doi:10.1128/MCB.00693-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Ribosomal DNA Transcription-Dependent Processes Interfere with Chromosome Segregation{dagger}

Brett N. Tomson,1 Damien D'Amours,2 Brittany S. Adamson,1 Luis Aragon,3 and Angelika Amon1*

Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, Massachusetts 02139,1 Institute for Research in Immunology and Cancer, Department of Pathology and Cell Biology, University of Montreal, P.O. Box 6128, Station Centre-Ville, Montreal, Quebec H3C 3J7, Canada,2 Cell Cycle Group, Clinical Sciences Centre, Medical Research Council, Imperial College London, Du Cane Road, London, London W12 0NN, United Kingdom3

Received 21 April 2006/ Accepted 26 May 2006

The ribosomal DNA (rDNA) is a specialized genomic region not only owing to its function as the nucleolar organizing region (NOR) but also because it is repetitive in nature and, at least in budding yeast, silenced for polymerase II (Pol II)-mediated transcription. Furthermore, cohesin-independent linkages hold the sister chromatids together at the rDNA loci, and their resolution requires the activity of the conserved protein phosphatase Cdc14. Here we show that rRNA transcription-dependent processes establish linkages at the rDNA, which affect segregation of this locus. Inactivation of Cfi1/Net1, a protein required for efficient rRNA transcription, or elimination of Pol I activity, which drives rRNA transcription, diminishes the need for CDC14 in rDNA segregation. Our results identify Pol I transcription-dependent processes as a novel means of establishing linkages between chromosomes.

* Corresponding author. Mailing address: Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139. Phone: (617) 258-8964. Fax: (617) 258-6558. E-mail: angelika{at}mit.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, August 2006, p. 6239-6247, Vol. 26, No. 16
0270-7306/06/$08.00+0     doi:10.1128/MCB.00693-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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