This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodríguez-Gabriel, M. A.
Right arrow Articles by Russell, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodríguez-Gabriel, M. A.
Right arrow Articles by Russell, P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2006, p. 6347-6356, Vol. 26, No. 17
0270-7306/06/$08.00+0     doi:10.1128/MCB.00286-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Upf1, an RNA Helicase Required for Nonsense-Mediated mRNA Decay, Modulates the Transcriptional Response to Oxidative Stress in Fission Yeast

Miguel A. Rodríguez-Gabriel,1,4 Stephen Watt,3 Jürg Bähler,3 and Paul Russell1,2*

Department of Molecular Biology,1 Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037,2 The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom,3 Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain4

Received 15 February 2006/ Returned for modification 14 March 2006/ Accepted 16 June 2006

In the fission yeast Schizosaccharomyces pombe, oxidative stress triggers the activation of the Spc1/Sty1 mitogen-activated protein kinase, which in turn phosphorylates the Atf1/Pcr1 heterodimeric transcription factor to effect global changes in the patterns of gene expression. This transcriptional response is also controlled by Csx1, an RNA-binding protein that directly associates with and stabilizes atf1+ mRNA. Here we report the surprising observation that this response also requires Upf1, a component of the nonsense-mediated mRNA decay (NMD) system. Accordingly, upf1{Delta} and csx1{Delta} strains are similarly sensitive to oxidative stress, and the effects of the mutations are not additive, suggesting that Upf1 and Csx1 work in the same pathway to stabilize atf1+ mRNA during oxidative stress. Consistent with these observations, whole-genome expression profiling studies have shown that Upf1 controls the expression of more than 100 genes that are transcriptionally induced in response to oxidative stress, the large majority of which are also controlled by Atf1 and Csx1. The unexpected connection between an NMD factor and the oxidative stress response in fission yeast may provide important new clues about the physiological function of NMD in other species.

* Corresponding author. Mailing address: Department of Molecular Biology, MB-3, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037. Phone: (858) 784-8273. Fax: (858) 784-2265. E-mail: prussell{at}scripps.edu.

Molecular and Cellular Biology, September 2006, p. 6347-6356, Vol. 26, No. 17
0270-7306/06/$08.00+0     doi:10.1128/MCB.00286-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Molina-Navarro, M. M., Castells-Roca, L., Belli, G., Garcia-Martinez, J., Marin-Navarro, J., Moreno, J., Perez-Ortin, J. E., Herrero, E. (2008). Comprehensive Transcriptional Analysis of the Oxidative Response in Yeast. J. Biol. Chem. 283: 17908-17918 [Abstract] [Full Text]  
  • Gardner, L. B. (2008). Hypoxic Inhibition of Nonsense-Mediated RNA Decay Regulates Gene Expression and the Integrated Stress Response. Mol. Cell. Biol. 28: 3729-3741 [Abstract] [Full Text]  
  • Oliveira, V., Romanow, W. J., Geisen, C., Otterness, D. M., Mercurio, F., Wang, H. G., Dalton, W. S., Abraham, R. T. (2008). A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-{alpha}-induced Apoptosis. J. Biol. Chem. 283: 13174-13184 [Abstract] [Full Text]  
  • Chen, D., Wilkinson, C. R.M., Watt, S., Penkett, C. J., Toone, W. M., Jones, N., Bahler, J. (2008). Multiple Pathways Differentially Regulate Global Oxidative Stress Responses in Fission Yeast. Mol. Biol. Cell 19: 308-317 [Abstract] [Full Text]  
  • Johansson, M. J. O., He, F., Spatrick, P., Li, C., Jacobson, A. (2007). Association of yeast Upf1p with direct substrates of the NMD pathway. Proc. Natl. Acad. Sci. USA 104: 20872-20877 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»