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Molecular and Cellular Biology, September 2006, p. 6557-6570, Vol. 26, No. 17
0270-7306/06/$08.00+0     doi:10.1128/MCB.00729-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Putative "Stemness" Gene Jam-B Is Not Required for Maintenance of Stem Cell State in Embryonic, Neural, or Hematopoietic Stem Cells

Takehisa Sakaguchi,1 Masazumi Nishimoto,2 Satoru Miyagi,3 Atsushi Iwama,3 Yohei Morita,4 Naoki Iwamori,4 Hiromitsu Nakauchi,4 Hiroshi Kiyonari,5 Masami Muramatsu,1 and Akihiko Okuda1,6*

Division of Developmental Biology,1 Radioisotope Experimental Laboratory, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan,2 Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan,3 Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan,4 Laboratory for Animal Resources and Genetic Engineering, RIKEN, Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan,5 REDS group, Saitama Small Enterprise Promotion Corporation, Skip City, Kawaguchi, Saitama 333-0844, Japan6

Received 27 April 2006/ Returned for modification 13 June 2006/ Accepted 20 June 2006

Many genes have been identified that are specifically expressed in multiple types of stem cells in their undifferentiated state. It is generally assumed that at least some of these putative "stemness" genes are involved in maintaining properties that are common to all stem cells. We compared gene expression profiles between undifferentiated and differentiated embryonic stem cells (ESCs) using DNA microarrays. We identified several genes with much greater signal in undifferentiated ESCs than in their differentiated derivatives, among them the putative stemness gene encoding junctional adhesion molecule B (Jam-B gene). However, in spite of the specific expression in undifferentiated ESCs, Jam-B mutant ESCs had normal morphology and pluripotency. Furthermore, Jam-B homozygous mutant mice are fertile and have no overt developmental defects. Moreover, we found that neural and hematopoietic stem cells recovered from Jam-B mutant mice are not impaired in their ability to self-renew and differentiate. These results demonstrate that Jam-B is dispensable for normal mouse development and stem cell identity in embryonic, neural, and hematopoietic stem cells.

* Corresponding author. Mailing address: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan. Phone: 81-429-85-7268. Fax: 011-81-429-85-7264. E-mail: akiokuda{at}saitama-med.ac.jp.

Molecular and Cellular Biology, September 2006, p. 6557-6570, Vol. 26, No. 17
0270-7306/06/$08.00+0     doi:10.1128/MCB.00729-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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