This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gearhart, M. D.
Right arrow Articles by Bardwell, V. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gearhart, M. D.
Right arrow Articles by Bardwell, V. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2006, p. 6880-6889, Vol. 26, No. 18
0270-7306/06/$08.00+0     doi:10.1128/MCB.00630-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Polycomb Group and SCF Ubiquitin Ligases Are Found in a Novel BCOR Complex That Is Recruited to BCL6 Targets{dagger}

Micah D. Gearhart,1 Connie M. Corcoran,1 Joseph A. Wamstad,2 and Vivian J. Bardwell1,2*

Department of Genetics, Cell Biology and Development and Cancer Center,1 Molecular, Cellular, Developmental Biology and Genetics Graduate Program, University of Minnesota, Minneapolis, Minnesota 554552

Received 11 April 2006/ Returned for modification 30 May 2006/ Accepted 26 June 2006

The corepressor BCOR potentiates transcriptional repression by the proto-oncoprotein BCL6 and suppresses the transcriptional activity of a common mixed-lineage leukemia fusion partner, AF9. Mutations in human BCOR cause male lethal, X-linked oculofaciocardiodental syndrome. We identified a BCOR complex containing Polycomb group (PcG) and Skp-Cullin-F-box subcomplexes. The PcG proteins include RING1, RYBP, NSPC1, a Posterior Sex Combs homolog, and RNF2, an E3 ligase for the mono-ubiquitylation of H2A. BCOR complex components and mono-ubiquitylated H2A localize to BCL6 targets, indicating that the BCOR complex employs PcG proteins to expand the repertoire of enzymatic activities that can be recruited by BCL6. This also suggests that BCL6 can target PcG proteins to DNA. In addition, the BCOR complex contains components of a second ubiquitin E3 ligase, namely, SKP1 and FBXL10 (JHDM1B). We show that BCOR coimmunoprecipitates isoforms of FBXL10 which contain a JmjC domain that recently has been determined to have histone H3K36 demethylase activity. The recruitment of two distinct classes of E3 ubiquitin ligases and a histone demethylase by BCOR suggests that BCOR uses a unique combination of epigenetic modifications to direct gene silencing.

* Corresponding author. Mailing address: Department of Genetics, Cell Biology and Development, 6-160 Jackson Hall, 321 Church St. SE, Minneapolis, MN 55455. Phone: (612) 626-7028. Fax: (612) 626-7031. E-mail: bardw001{at}umn.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, September 2006, p. 6880-6889, Vol. 26, No. 18
0270-7306/06/$08.00+0     doi:10.1128/MCB.00630-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schuettengruber, B., Cavalli, G. (2009). Recruitment of Polycomb group complexes and their role in the dynamic regulation of cell fate choice. Development 136: 3531-3542 [Abstract] [Full Text]  
  • Tzatsos, A., Pfau, R., Kampranis, S. C., Tsichlis, P. N. (2009). Ndy1/KDM2B immortalizes mouse embryonic fibroblasts by repressing the Ink4a/Arf locus. Proc. Natl. Acad. Sci. USA 106: 2641-2646 [Abstract] [Full Text]  
  • Polytarchou, C., Pfau, R., Hatziapostolou, M., Tsichlis, P. N. (2008). The JmjC Domain Histone Demethylase Ndy1 Regulates Redox Homeostasis and Protects Cells from Oxidative Stress. Mol. Cell. Biol. 28: 7451-7464 [Abstract] [Full Text]  
  • Lagarou, A., Mohd-Sarip, A., Moshkin, Y. M., Chalkley, G. E., Bezstarosti, K., Demmers, J. A.A., Verrijzer, C. P. (2008). dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing. Genes Dev. 22: 2799-2810 [Abstract] [Full Text]  
  • Gonzalez, I., Aparicio, R., Busturia, A. (2008). Functional Characterization of the dRYBP Gene in Drosophila. Genetics 179: 1373-1388 [Abstract] [Full Text]  
  • Wu, X., Gong, Y., Yue, J., Qiang, B., Yuan, J., Peng, X. (2008). Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. Nucleic Acids Res 36: 3590-3599 [Abstract] [Full Text]  
  • Cloos, P. A.C., Christensen, J., Agger, K., Helin, K. (2008). Erasing the methyl mark: histone demethylases at the center of cellular differentiation and disease. Genes Dev. 22: 1115-1140 [Abstract] [Full Text]  
  • Mendez, L. M., Polo, J. M., Yu, J. J., Krupski, M., Ding, B. B., Melnick, A., Ye, B. H. (2008). CtBP Is an Essential Corepressor for BCL6 Autoregulation. Mol. Cell. Biol. 28: 2175-2186 [Abstract] [Full Text]  
  • Yamagishi, T., Hirose, S., Kondo, T. (2008). Secondary DNA structure formation for Hoxb9 promoter and identification of its specific binding protein. Nucleic Acids Res 36: 1965-1975 [Abstract] [Full Text]  
  • Cales, C., Roman-Trufero, M., Pavon, L., Serrano, I., Melgar, T., Endoh, M., Perez, C., Koseki, H., Vidal, M. (2008). Inactivation of the Polycomb Group Protein Ring1B Unveils an Antiproliferative Role in Hematopoietic Cell Expansion and Cooperation with Tumorigenesis Associated with Ink4a Deletion. Mol. Cell. Biol. 28: 1018-1028 [Abstract] [Full Text]  
  • Hilton, E. N., Manson, F. D.C., Urquhart, J. E., Johnston, J. J., Slavotinek, A. M., Hedera, P., Stattin, E.-L., Nordgren, A., Biesecker, L. G., Black, G. C.M. (2007). Left-sided embryonic expression of the BCL-6 corepressor, BCOR, is required for vertebrate laterality determination. Hum Mol Genet 16: 1773-1782 [Abstract] [Full Text]  
  • Pagan, J. K., Arnold, J., Hanchard, K. J., Kumar, R., Bruno, T., Jones, M. J. K., Richard, D. J., Forrest, A., Spurdle, A., Verdin, E., Crossley, M., Fanciulli, M., Chenevix-Trench, G., Young, D. B., Khanna, K. K. (2007). A Novel Corepressor, BCoR-L1, Represses Transcription through an Interaction with CtBP. J. Biol. Chem. 282: 15248-15257 [Abstract] [Full Text]  
  • Nielsen, J. V., Nielsen, F. H., Ismail, R., Noraberg, J., Jensen, N. A. (2007). Hippocampus-like corticoneurogenesis induced by two isoforms of the BTB-zinc finger gene Zbtb20 in mice. Development 134: 1133-1140 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»