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Molecular and Cellular Biology, September 2006, p. 6880-6889, Vol. 26, No. 18
0270-7306/06/$08.00+0     doi:10.1128/MCB.00630-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Polycomb Group and SCF Ubiquitin Ligases Are Found in a Novel BCOR Complex That Is Recruited to BCL6 Targets{dagger}

Micah D. Gearhart,1 Connie M. Corcoran,1 Joseph A. Wamstad,2 and Vivian J. Bardwell1,2*

Department of Genetics, Cell Biology and Development and Cancer Center,1 Molecular, Cellular, Developmental Biology and Genetics Graduate Program, University of Minnesota, Minneapolis, Minnesota 554552

Received 11 April 2006/ Returned for modification 30 May 2006/ Accepted 26 June 2006

The corepressor BCOR potentiates transcriptional repression by the proto-oncoprotein BCL6 and suppresses the transcriptional activity of a common mixed-lineage leukemia fusion partner, AF9. Mutations in human BCOR cause male lethal, X-linked oculofaciocardiodental syndrome. We identified a BCOR complex containing Polycomb group (PcG) and Skp-Cullin-F-box subcomplexes. The PcG proteins include RING1, RYBP, NSPC1, a Posterior Sex Combs homolog, and RNF2, an E3 ligase for the mono-ubiquitylation of H2A. BCOR complex components and mono-ubiquitylated H2A localize to BCL6 targets, indicating that the BCOR complex employs PcG proteins to expand the repertoire of enzymatic activities that can be recruited by BCL6. This also suggests that BCL6 can target PcG proteins to DNA. In addition, the BCOR complex contains components of a second ubiquitin E3 ligase, namely, SKP1 and FBXL10 (JHDM1B). We show that BCOR coimmunoprecipitates isoforms of FBXL10 which contain a JmjC domain that recently has been determined to have histone H3K36 demethylase activity. The recruitment of two distinct classes of E3 ubiquitin ligases and a histone demethylase by BCOR suggests that BCOR uses a unique combination of epigenetic modifications to direct gene silencing.

* Corresponding author. Mailing address: Department of Genetics, Cell Biology and Development, 6-160 Jackson Hall, 321 Church St. SE, Minneapolis, MN 55455. Phone: (612) 626-7028. Fax: (612) 626-7031. E-mail: bardw001{at}umn.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, September 2006, p. 6880-6889, Vol. 26, No. 18
0270-7306/06/$08.00+0     doi:10.1128/MCB.00630-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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