This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leeds, N. B.
Right arrow Articles by Staley, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leeds, N. B.
Right arrow Articles by Staley, J. P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2006, p. 513-522, Vol. 26, No. 2
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.2.513-522.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Splicing Factor Prp43p, a DEAH Box ATPase, Functions in Ribosome Biogenesis

Nina B. Leeds ,1,{dagger},{ddagger} Eliza C. Small,2,{ddagger} Shawna L. Hiley,3 Timothy R. Hughes,3,4 and Jonathan P. Staley1*

Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637,1 Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637,2 Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, Ontario M5G 1L6, Canada,3 Department of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada4

Received 14 June 2005/ Returned for modification 13 July 2005/ Accepted 20 October 2005

Biogenesis of the small and large ribosomal subunits requires modification, processing, and folding of pre-rRNA to yield mature rRNA. Here, we report that efficient biogenesis of both small- and large-subunit rRNAs requires the DEAH box ATPase Prp43p, a pre-mRNA splicing factor. By steady-state analysis, a cold-sensitive prp43 mutant accumulates 35S pre-rRNA and depletes 20S, 27S, and 7S pre-rRNAs, precursors to the small- and large-subunit rRNAs. By pulse-chase analysis, the prp43 mutant is defective in the formation of 20S and 27S pre-rRNAs and in the accumulation of 18S and 25S mature rRNAs. Wild-type Prp43p immunoprecipitates pre-rRNAs and mature rRNAs, indicating a direct role in ribosome biogenesis. The Prp43p-Q423N mutant immunoprecipitates 27SA2 pre-rRNA threefold more efficiently than the wild type, suggesting a critical role for Prp43p at the earliest stages of large-subunit biogenesis. Consistent with an early role for Prp43p in ribosome biogenesis, Prp43p immunoprecipitates the majority of snoRNAs; further, compared to the wild type, the prp43 mutant generally immunoprecipitates the snoRNAs more efficiently. In the prp43 mutant, the snoRNA snR64 fails to methylate residue C2337 in 27S pre-rRNA, suggesting a role in snoRNA function. We propose that Prp43p promotes recycling of snoRNAs and biogenesis factors during pre-rRNA processing, similar to its recycling role in pre-mRNA splicing. The dual function for Prp43p in the cell raises the possibility that ribosome biogenesis and pre-mRNA splicing may be coordinately regulated.

* Corresponding author. Mailing address: University of Chicago, 920 E. 58th St., Chicago, IL 60637. Phone: (773) 834-5886. Fax: (773) 834-9064. E-mail: jstaley{at}uchicago.edu.

{dagger} Current address: Skirball Institute for Biomolecular Medicine, Lab 4-9, 540 First Ave., New York, NY 10016.

{ddagger} These authors contributed equally to this work.

Molecular and Cellular Biology, January 2006, p. 513-522, Vol. 26, No. 2
0022-538X/06/$08.00+0     doi:10.1128/MCB.26.2.513-522.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pandit, S., Paul, S., Zhang, L., Chen, M., Durbin, N., Harrison, S. M. W., Rymond, B. C. (2009). Spp382p Interacts with Multiple Yeast Splicing Factors, Including Possible Regulators of Prp43 DExD/H-Box Protein Function. Genetics 183: 195-206 [Abstract] [Full Text]  
  • Zhao, R., Kakihara, Y., Gribun, A., Huen, J., Yang, G., Khanna, M., Costanzo, M., Brost, R. L., Boone, C., Hughes, T. R., Yip, C. M., Houry, W. A. (2008). Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation. JCB 180: 563-578 [Abstract] [Full Text]  
  • Tsai, R.-T., Tseng, C.-K., Lee, P.-J., Chen, H.-C., Fu, R.-H., Chang, K.-j., Yeh, F.-L., Cheng, S.-C. (2007). Dynamic Interactions of Ntr1-Ntr2 with Prp43 and with U5 Govern the Recruitment of Prp43 To Mediate Spliceosome Disassembly. Mol. Cell. Biol. 27: 8027-8037 [Abstract] [Full Text]  
  • Tanaka, N., Aronova, A., Schwer, B. (2007). Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Genes Dev. 21: 2312-2325 [Abstract] [Full Text]  
  • Chung, T., Kim, C. S., Nguyen, H. N., Meeley, R. B., Larkins, B. A. (2007). The Maize Zmsmu2 Gene Encodes a Putative RNA-Splicing Factor That Affects Protein Synthesis and RNA Processing during Endosperm Development. Plant Physiol. 144: 821-835 [Abstract] [Full Text]  
  • Tijerina, P., Bhaskaran, H., Russell, R. (2006). Nonspecific binding to structured RNA and preferential unwinding of an exposed helix by the CYT-19 protein, a DEAD-box RNA chaperone. Proc. Natl. Acad. Sci. USA 103: 16698-16703 [Abstract] [Full Text]  
  • Liang, X.-h., Fournier, M. J. (2006). The Helicase Has1p Is Required for snoRNA Release from Pre-rRNA. Mol. Cell. Biol. 26: 7437-7450 [Abstract] [Full Text]  
  • Boon, K.-L., Auchynnikava, T., Edwalds-Gilbert, G., Barrass, J. D., Droop, A. P., Dez, C., Beggs, J. D. (2006). Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.. Mol. Cell. Biol. 26: 6016-6023 [Abstract] [Full Text]  
  • Bleichert, F., Granneman, S., Osheim, Y. N., Beyer, A. L., Baserga, S. J. (2006). The PINc domain protein Utp24, a putative nuclease, is required for the early cleavage steps in 18S rRNA maturation. Proc. Natl. Acad. Sci. USA 103: 9464-9469 [Abstract] [Full Text]  
  • Combs, D. J., Nagel, R. J., Ares, M. Jr., Stevens, S. W. (2006). Prp43p Is a DEAH-Box Spliceosome Disassembly Factor Essential for Ribosome Biogenesis. Mol. Cell. Biol. 26: 523-534 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»