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Molecular and Cellular Biology, October 2006, p. 7388-7396, Vol. 26, No. 20
0270-7306/06/$08.00+0     doi:10.1128/MCB.01159-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of ISW2 by Concerted Action of Histone H4 Tail and Extranucleosomal DNA

Weiwei Dang ,{dagger},§ Mohamedi N. Kagalwala,{dagger},{ddagger} and Blaine Bartholomew*

Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901-4413

Received 27 June 2006/ Accepted 24 July 2006

The stable contact of ISW2 with nucleosomal DNA ~20 bp from the dyad was shown by DNA footprinting and photoaffinity labeling using recombinant histone octamers to require the histone H4 N-terminal tail. Efficient ISW2 remodeling also required the H4 N-terminal tail, although the lack of the H4 tail can be mostly compensated for by increasing the incubation time or concentration of ISW2. Similarly, the length of extranucleosomal DNA affected the stable contact of ISW2 with this same internal nucleosomal site, with the optimal length being 70 to 85 bp. These data indicate the histone H4 tail, in concert with a favorable length of extranucleosomal DNA, recruits and properly orients ISW2 onto the nucleosome for efficient nucleosome remodeling. One consequence of this property of ISW2 is likely its previously observed nucleosome spacing activity.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Dr., Neckers Bldg., Room 229, Carbondale, IL 62901-4413. Phone: (618) 453-6437. Fax: (618) 453-6440. E-mail: bbartholomew{at}siumed.edu.

{dagger} W.D. and M.N.K. have made equal contributions to this work.

§ Present address: The Wistar Institute, 3601 Spruce St., Room 201, Philadelphia, PA 19104.

{ddagger} Present address: Department of Molecular Genetics, Section of Cancer Genetics, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030.

Molecular and Cellular Biology, October 2006, p. 7388-7396, Vol. 26, No. 20
0270-7306/06/$08.00+0     doi:10.1128/MCB.01159-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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