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Molecular and Cellular Biology, October 2006, p. 7430-7436, Vol. 26, No. 20
0270-7306/06/$08.00+0     doi:10.1128/MCB.00624-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Redox Regulation of Human OGG1 Activity in Response to Cellular Oxidative Stress{triangledown}

Anne Bravard,1,2 Monique Vacher,1,2 Barbara Gouget,3 Alexandre Coutant,1 Florence Hillairet de Boisferon,1,2 Stéphanie Marsin,1,2 Sylvie Chevillard,1 and J. Pablo Radicella1,2*

Département de Radiobiologie et Radiopathologie, Commissariat à l'Energie Atomique,1 UMR 217 CNRS/CEA, 18 route du Panorama, F-92265 Fontenay aux Roses, France,2 Laboratoire Pierre Sue CEA-CNRS UMR 9956, F-91191 Gif sur Yvette, France3

Received 11 April 2006/ Returned for modification 5 June 2006/ Accepted 31 July 2006

8-Oxoguanine (8-oxoG), a common and mutagenic form of oxidized guanine in DNA, is eliminated mainly through base excision repair. In human cells its repair is initiated by human OGG1 (hOGG1), an 8-oxoG DNA glycosylase. We investigated the effects of an acute cadmium exposure of human lymphoblastoid cells on the activity of hOGG1. We show that coinciding with alteration of the redox cellular status, the 8-oxoG DNA glycosylase activity of hOGG1 was nearly completely inhibited. However, the hOGG1 activity returned to normal levels once the redox cellular status was normalized. In vitro, the activity of purified hOGG1 was abolished by cadmium and could not be recovered by EDTA. In cells, however, the reversible inactivation of OGG1 activity by cadmium was strictly associated with reversible oxidation of the protein. Moreover, the 8-oxoG DNA glycosylase activity of purified OGG1 and that from crude extracts were modulated by cysteine-modifying agents. Oxidation of OGG1 by the thiol oxidant diamide led to inhibition of the activity and a protein migration pattern similar to that seen in cadmium-treated cells. These results suggest that cadmium inhibits hOGG1 activity mainly by indirect oxidation of critical cysteine residues and that excretion of the metal from the cells leads to normalization of the redox cell status and restoration of an active hOGG1. The results presented here unveil a novel redox-dependent mechanism for the regulation of OGG1 activity.

* Corresponding author. Mailing address: Département de Radiobiologie et Radiopathologie, CEA, 18 route du Panorama, F-92265 Fontenay aux Roses, France. Phone: 33 1 46 54 88 57. Fax: 33 1 46 54 88 59. E-mail: jpradicella{at}cea.fr.

{triangledown} Published ahead of print on 21 August 2006.

Molecular and Cellular Biology, October 2006, p. 7430-7436, Vol. 26, No. 20
0270-7306/06/$08.00+0     doi:10.1128/MCB.00624-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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