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Molecular and Cellular Biology, October 2006, p. 7451-7465, Vol. 26, No. 20
0270-7306/06/$08.00+0     doi:10.1128/MCB.00684-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

An hpr1 Point Mutation That Impairs Transcription and mRNP Biogenesis without Increasing Recombination{triangledown}

Pablo Huertas, María L. García-Rubio, Ralf E. Wellinger, Rosa Luna, and Andrés Aguilera*

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avd. Reina Mercedes 6, 41012 Sevilla, Spain

Received 20 April 2006/ Returned for modification 13 June 2006/ Accepted 4 August 2006

THO/TREX, a conserved eukaryotic protein complex, is a key player at the interface between transcription and mRNP metabolism. The lack of a functional THO complex impairs transcription, leads to transcription-dependent hyperrecombination, causes mRNA export defects and fast mRNA decay, and retards replication fork progression in a transcription-dependent manner. To get more insight into the interconnection between mRNP biogenesis and genomic instability, we searched for HPR1 mutations that differentially affect gene expression and recombination. We isolated mutants that were barely affected in gene expression but exhibited a hyperrecombination phenotype. In addition, we isolated a mutant, hpr1-101, with a strong defect in transcription, as observed for lacZ, and a general defect in mRNA export that did not display a relevant hyperrecombination phenotype. In THO single-null mutants, but not in the hpr1 point mutants studied, THO and its subunits were unstable. Interestingly, in contrast to hyperrecombinant null mutants, hpr1-101 did not cause retardation of replication fork progression. Transcription and mRNP biogenesis can therefore be impaired by THO/TREX dysfunction without increasing recombination, suggesting that it is possible to separate the mechanism(s) responsible for mRNA biogenesis defects from the further step of triggering transcription-dependent recombination.

* Corresponding author. Mailing address: CABIMER, Av. Americo Vespucio s/n, 41092 Seville, Spain. Phone: 34 954 468372. Fax: 34 954 461664. E-mail: aguilo{at}us.es.

{triangledown} Published ahead of print on 14 August 2006.

Molecular and Cellular Biology, October 2006, p. 7451-7465, Vol. 26, No. 20
0270-7306/06/$08.00+0     doi:10.1128/MCB.00684-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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