This Article Full Text Full Text (PDF) Other Versions of this Article: MCB.00870-06v1 26/21/7858    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dieppois, G. Articles by Stutz, F. Search for Related Content PubMed PubMed Citation Articles by Dieppois, G. Articles by Stutz, F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2006, p. 7858-7870, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00870-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cotranscriptional Recruitment to the mRNA Export Receptor Mex67p Contributes to Nuclear Pore Anchoring of Activated Genes

Guennaelle Dieppois, Nahid Iglesias, and Françoise Stutz^*

Department of Cell Biology, Sciences III, 30 Quai E. Ansermet, 1211 Geneva 4, Switzerland

Received 16 May 2006/ Returned for modification 14 June 2006/ Accepted 24 August 2006

Transcription activation of some Saccharomyces cerevisiae genes is paralleled by their repositioning to the nuclear periphery, but the mechanism underlying gene anchoring is poorly defined. We show that the nuclear pore complex-associated Mlp1p and the shuttling mRNA export receptor Mex67p contribute to the stable association of the activated GAL10 and HSP104 genes with the nuclear periphery. However, we find no obligatory link between gene positioning and gene expression. Furthermore, gene anchoring correlates with the cotranscriptional recruitment of Mex67p to transcribing genes. Notably, the association of Mex67p with chromatin is not mediated by RNA. Interestingly, a mutant GAL2 gene lacking the coding region is still able to recruit Mex67p upon transcriptional activation and to relocate to the nuclear periphery. Together these data suggest that, at least for GAL2, nascent messenger ribonucleoprotein does not play a major role in gene anchoring and that the early recruitment of Mex67p contributes to gene repositioning by virtue of an RNA-independent process.

Published ahead of print on 5 September 2006.

These authors contributed equally to this work.

This article has been cited by other articles:

• Thomsen, R., Saguez, C., Nasser, T., Jensen, T. H. (2008). General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants. RNA 14: 706-716 [Abstract] [Full Text]  
• Brown, C. R., Kennedy, C. J., Delmar, V. A., Forbes, D. J., Silver, P. A. (2008). Global histone acetylation induces functional genomic reorganization at mammalian nuclear pore complexes. Genes Dev. 22: 627-639 [Abstract] [Full Text]  
• Palancade, B., Liu, X., Garcia-Rubio, M., Aguilera, A., Zhao, X., Doye, V. (2007). Nucleoporins Prevent DNA Damage Accumulation by Modulating Ulp1-dependent Sumoylation Processes. Mol. Biol. Cell 18: 2912-2923 [Abstract] [Full Text]  
• Gaillard, H., Wellinger, R. E., Aguilera, A. (2007). A new connection of mRNP biogenesis and export with transcription-coupled repair. Nucleic Acids Res 35: 3893-3906 [Abstract] [Full Text]  
• Luthra, R., Kerr, S. C., Harreman, M. T., Apponi, L. H., Fasken, M. B., Ramineni, S., Chaurasia, S., Valentini, S. R., Corbett, A. H. (2007). Actively Transcribed GAL Genes Can Be Physically Linked to the Nuclear Pore by the SAGA Chromatin Modifying Complex. J. Biol. Chem. 282: 3042-3049 [Abstract] [Full Text]