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Molecular and Cellular Biology, November 2006, p. 8011-8021, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.01055-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Coatomer, the Coat Protein of COPI Transport Vesicles, Discriminates Endoplasmic Reticulum Residents from p24 Proteins{triangledown}

Julien Béthune,* Matthijs Kol, Julia Hoffmann, Inge Reckmann, Britta Brügger, and Felix Wieland*

Biochemie-Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany

Received 12 June 2006/ Returned for modification 3 August 2006/ Accepted 17 August 2006

In the formation of COPI vesicles, interactions take place between the coat protein coatomer and membrane proteins: either cargo proteins for retrieval to the endoplasmic reticulum (ER) or proteins that cycle between the ER and the Golgi. While the binding sites on coatomer for ER residents have been characterized, how cycling proteins bind to the COPI coat is still not clear. In order to understand at a molecular level the mechanism of uptake of such proteins, we have investigated the binding to coatomer of p24 proteins as examples of cycling proteins as well as that of ER-resident cargos. The p24 proteins required dimerization to interact with coatomer at two independent binding sites in {gamma}-COP. In contrast, ER-resident cargos bind to coatomer as monomers and to sites other than {gamma}-COP. The COPI coat therefore discriminates between p24 proteins and ER-resident proteins by differential binding involving distinct subunits.

* Corresponding author. Mailing address: Biochemie-Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany. Phone for Julien Béthune: 49-6221-544688. Fax: 49-6221-544366. E-mail: Julien.Bethune{at}bzh.uni-heidelberg.de. Phone for Felix Wieland: 49-6221-544150. Fax: 49-6221-544366. E-mail: Felix.Wieland{at}bzh.uni-heidelberg.de.

{triangledown} Published ahead of print on 28 August 2006.

Molecular and Cellular Biology, November 2006, p. 8011-8021, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.01055-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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