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Molecular and Cellular Biology, November 2006, p. 8293-8302, Vol. 26, No. 22
0270-7306/06/$08.00+0     doi:10.1128/MCB.02455-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Control of G₂/M Transition by Drosophila Fos ^,

Joogyung Hyun,¹ Isabelle Bécam,^2, Constantin Yanicostas,² and Dirk Bohmann^1*

Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, New York,¹ Institut Jacques Monod, 2 Place Jussieu, Paris F-75251, France²

Received 22 December 2005/ Returned for modification 3 February 2006/ Accepted 29 August 2006

The transcription factors of the Fos family have long been associated with the control of cell proliferation, although the molecular and cellular mechanisms that mediate this function are poorly understood. We investigated the contributions of Fos to the cell cycle and cell growth control using Drosophila imaginal discs as a genetically accessible system. The RNA interference-mediated inhibition of Fos in proliferating cells of the wing and eye discs resulted in a specific defect in the G₂-to-M-phase transition, while cell growth remained unimpaired, resulting in a marked reduction in organ size. Consistent with the conclusion that Fos is required for mitosis, we identified cyclin B as a direct transcriptional target of Fos in Drosophila melanogaster, with Fos binding to a region upstream of the cyclin B gene in vivo and cyclin B mRNA being specifically reduced under Fos loss-of-function conditions.

Published ahead of print on 11 September 2006.

Supplemental material for this article may be found at http://mcb.asm.org/.

Present address: ICREA and Institut de Recerca Biomedica, Parc Cientific de Barcelona, Josep Samitier, 1-5, 08028 Barcelona, Spain.