This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, Y.
Right arrow Articles by Clark, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, Y.
Right arrow Articles by Clark, D. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2006, p. 8607-8622, Vol. 26, No. 22
0270-7306/06/$08.00+0     doi:10.1128/MCB.00678-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Activation of Saccharomyces cerevisiae HIS3 Results in Gcn4p-Dependent, SWI/SNF-Dependent Mobilization of Nucleosomes over the Entire Gene{triangledown}

Yeonjung Kim,1,{dagger} Neil McLaughlin,1 Kim Lindstrom,2 Toshio Tsukiyama,2 and David J. Clark1*

Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Building 6A, Room 2A14, 6 Center Drive, Bethesda, Maryland 20892,1 Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 981092

Received 19 April 2006/ Returned for modification 30 May 2006/ Accepted 3 September 2006

The effects of transcriptional activation on the chromatin structure of the Saccharomyces cerevisiae HIS3 gene were addressed by mapping the precise positions of nucleosomes in uninduced and induced chromatin. In the absence of the Gcn4p activator, the HIS3 gene is organized into a predominant nucleosomal array. In wild-type chromatin, this array is disrupted, and several alternative overlapping nucleosomal arrays are formed. The disruption of the predominant array also requires the SWI/SNF remodeling machine, indicating that the SWI/SNF complex plays an important role in nucleosome mobilization over the entire HIS3 gene. The Isw1 remodeling complex plays a more subtle role in determining nucleosome positions on HIS3, favoring positions different from those preferred by the SWI/SNF complex. Both the SWI/SNF and Isw1 complexes are constitutively present in HIS3 chromatin, although Isw1 tends to be excluded from the HIS3 promoter. Despite the apparent disorder of HIS3 chromatin generated by the formation of multiple nucleosomal arrays, nucleosome density profiles indicate that some long-range order is always present. We propose that Gcn4p stimulates nucleosome mobilization over the entire HIS3 gene by the SWI/SNF complex. We suggest that the net effect of interplay among remodeling machines at HIS3 is to create a highly dynamic chromatin structure.

* Corresponding author. Mailing address: Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Building 6A, Room 2A14, 6 Center Drive, Bethesda, MD 20892. Phone: (301) 496-6966. Fax: (301) 480-1907. E-mail: clarkda{at}mail.nih.gov.

{triangledown} Published ahead of print on 18 September 2006.

{dagger} Present address: Division of Structural and Functional Genomics, Center for Genome Science, National Institute of Health, KCDC, Seoul, South Korea.

Molecular and Cellular Biology, November 2006, p. 8607-8622, Vol. 26, No. 22
0270-7306/06/$08.00+0     doi:10.1128/MCB.00678-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Caserta, M., Agricola, E., Churcher, M., Hiriart, E., Verdone, L., Di Mauro, E., Travers, A. (2009). A translational signature for nucleosome positioning in vivo. Nucleic Acids Res 37: 5309-5321 [Abstract] [Full Text]  
  • Visone, T., Charron, M., Wright, W. W. (2009). Activation and Repression Domains Within the Promoter of the Rat Cathepsin L Gene Orchestrate Sertoli Cell-Specific and Stage-Specific Gene Transcription in Transgenic Mice. Biol. Reprod. 81: 571-579 [Abstract] [Full Text]  
  • Gkikopoulos, T., Havas, K. M., Dewar, H., Owen-Hughes, T. (2009). SWI/SNF and Asf1p Cooperate To Displace Histones during Induction of the Saccharomyces cerevisiae HO Promoter. Mol. Cell. Biol. 29: 4057-4066 [Abstract] [Full Text]  
  • Joo, Y. J., Kim, J.-A., Baek, J. H., Seong, K. M., Han, K.-D., Song, J. M., Choi, J. Y., Kim, J. (2009). Cooperative Regulation of ADE3 Transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae. Eukaryot Cell 8: 1268-1277 [Abstract] [Full Text]  
  • Zawadzki, K. A., Morozov, A. V., Broach, J. R. (2009). Chromatin-dependent Transcription Factor Accessibility Rather than Nucleosome Remodeling Predominates during Global Transcriptional Restructuring in Saccharomyces cerevisiae. Mol. Biol. Cell 20: 3503-3513 [Abstract] [Full Text]  
  • Pinskaya, M., Nair, A., Clynes, D., Morillon, A., Mellor, J. (2009). Nucleosome Remodeling and Transcriptional Repression Are Distinct Functions of Isw1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 29: 2419-2430 [Abstract] [Full Text]  
  • Adkins, M. W., Williams, S. K., Linger, J., Tyler, J. K. (2007). Chromatin Disassembly from the PHO5 Promoter Is Essential for the Recruitment of the General Transcription Machinery and Coactivators. Mol. Cell. Biol. 27: 6372-6382 [Abstract] [Full Text]  
  • Fleming, A. B., Pennings, S. (2007). Tup1-Ssn6 and Swi-Snf remodelling activities influence long-range chromatin organization upstream of the yeast SUC2 gene. Nucleic Acids Res 0: gkm573v1-12 [Abstract] [Full Text]  
  • Koehler, R. N., Rachfall, N., Rolfes, R. J. (2007). Activation of the ADE Genes Requires the Chromatin Remodeling Complexes SAGA and SWI/SNF. Eukaryot Cell 6: 1474-1485 [Abstract] [Full Text]  
  • Bert, A. G., Johnson, B. V., Baxter, E. W., Cockerill, P. N. (2007). A Modular Enhancer Is Differentially Regulated by GATA and NFAT Elements That Direct Different Tissue-Specific Patterns of Nucleosome Positioning and Inducible Chromatin Remodeling. Mol. Cell. Biol. 27: 2870-2885 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»