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Molecular and Cellular Biology, December 2006, p. 8964-8975, Vol. 26, No. 23
0270-7306/06/$08.00+0     doi:10.1128/MCB.00670-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Ccpg1, a Novel Scaffold Protein That Regulates the Activity of the Rho Guanine Nucleotide Exchange Factor Dbs{triangledown}

Elena V. Kostenko, Oyenike O. Olabisi, Sutapa Sahay, Pedro L. Rodriguez, and Ian P. Whitehead*

Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, and New Jersey Medical School-University Hospital Cancer Center of UMDNJ, Newark, New Jersey 07101-1709

Received 18 April 2006/ Returned for modification 26 May 2006/ Accepted 18 September 2006

Dbs is a Rho-specific guanine nucleotide exchange factor (RhoGEF) with in vitro exchange activity specific for RhoA and Cdc42. Like many RhoGEF family members, the in vivo exchange activity of Dbs is restricted in a cell-specific manner. Here we report the characterization of a novel scaffold protein (designated cell cycle progression protein 1 [Ccpg1]) that interacts with Dbs and modulates its in vivo exchange specificity. When coexpressed in mammalian cells, Ccpg1 binds to the Dbl homology/pleckstrin homology domain tandem motif of Dbs and inhibits its exchange activity toward RhoA, but not Cdc42. Expression of Ccpg1 correlates with the ability of Dbs to activate endogenous RhoA in cultured cells, and suppression of endogenous Ccpg1 expression potentiates Dbs exchange activity toward RhoA. The isolated Dbs binding domain of Ccpg1 is not sufficient to suppress Dbs exchange activity on RhoA, thus suggesting a regulatory interaction. Ccpg1 mediates recruitment of endogenous Src kinase into Dbs-containing complexes and interacts with the Rho family member Cdc42. Collectively, our studies suggest that Ccpg1 represents a new class of regulatory scaffold protein that can function as both an assembly platform for Rho protein signaling complexes and a regulatory protein which can restrict the substrate utilization of a promiscuous RhoGEF family member.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ 07101-1709. Phone: (973) 972-4483, ext. 25215. Fax: (973) 972-3644. E-mail: whiteip{at}umdnj.edu.

{triangledown} Published ahead of print on 25 September 2006.

Molecular and Cellular Biology, December 2006, p. 8964-8975, Vol. 26, No. 23
0270-7306/06/$08.00+0     doi:10.1128/MCB.00670-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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