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Molecular and Cellular Biology, December 2006, p. 8984-8991, Vol. 26, No. 23
0270-7306/06/$08.00+0     doi:10.1128/MCB.00959-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mice Mutant in the DM Domain Gene Dmrt4 Are Viable and Fertile but Have Polyovular Follicles{triangledown}

Jorune Balciuniene, Vivian J. Bardwell, and David Zarkower*

Department of Genetics, Cell, and Developmental Biology, University of Minnesota, Minneapolis, Minnesota 55455

Received 30 May 2006/ Returned for modification 26 June 2006/ Accepted 9 September 2006

Proteins containing the DM domain, a zinc finger-like DNA binding motif, have been implicated in sexual differentiation in diverse metazoan organisms. Of seven mammalian DM domain genes, only Dmrt1 and Dmrt2 have been functionally analyzed. Here, we report expression analysis and targeted disruption of Dmrt4 (also called DmrtA1) in the mouse. Dmrt4 is widely expressed during embryonic and postnatal development. However, we find that mice homozygous for a putative null mutation in Dmrt4 develop essentially normally, undergo full sexual differentiation in both sexes, and are fertile. We observed two potential mutant phenotypes in Dmrt4 mutant mice. First, ovaries of most mutant females have polyovular follicles, suggesting a role in folliculogenesis. Second, 25% of mutant males consistently exhibited copulatory behavior toward other males. We also tested potential redundancy between Dmrt4 and two other gonadally expressed DM domain genes, Dmrt1 and Dmrt7. We observed no enhancement of gonadal phenotypes in the double mutants, suggesting that these genes function independently in gonadal development.

* Corresponding author. Mailing address: Department of Genetics Cell Biology and Development, 6-160 Jackson Hall, 321 Church St. SE, Minneapolis, MN 55455. Phone: (612) 625-9450. Fax: (612) 626-7031. E-mail: zarko001{at}umn.edu.

{triangledown} Published ahead of print on 18 September 2006.

Molecular and Cellular Biology, December 2006, p. 8984-8991, Vol. 26, No. 23
0270-7306/06/$08.00+0     doi:10.1128/MCB.00959-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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