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Molecular and Cellular Biology, December 2006, p. 9149-9161, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.00310-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Spindle Assembly Checkpoint Regulates the Phosphorylation State of a Subset of DNA Checkpoint Proteins in Saccharomyces cerevisiae{triangledown}

Céline Clémenson and Marie-Claude Marsolier-Kergoat*

Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, 91191 Gif-sur-Yvette, France

Received 20 February 2006/ Returned for modification 17 April 2006/ Accepted 3 October 2006

The DNA and the spindle assembly checkpoints play key roles in maintaining genomic integrity by coordinating cell responses to DNA lesions and spindle dysfunctions, respectively. These two surveillance pathways seem to operate mostly independently of one another, and little is known about their potential physiological connections. Here, we show that in Saccharomyces cerevisiae, the activation of the spindle assembly checkpoint triggers phosphorylation changes in two components of the DNA checkpoint, Rad53 and Rad9. These modifications are independent of the other DNA checkpoint proteins and are abolished in spindle checkpoint-defective mutants, hinting at specific functions for Rad53 and Rad9 in the spindle damage response. Moreover, we found that after UV irradiation, Rad9 phosphorylation is altered and Rad53 inactivation is accelerated when the spindle checkpoint is activated, which suggests the implication of the spindle checkpoint in the regulation of the DNA damage response.

* Corresponding author. Mailing address: Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, 91191 Gif-sur-Yvette, France. Phone: 00-33-1-69-08-83-54. Fax: 00-33-1-69-08-47-12. E-mail: mcmk{at}cea.fr.

{triangledown} Published ahead of print on 23 October 2006.

Molecular and Cellular Biology, December 2006, p. 9149-9161, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.00310-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lancelot, N., Charier, G., Couprie, J., Duband-Goulet, I., Alpha-Bazin, B., Quemeneur, E., Ma, E., Marsolier-Kergoat, M.-C., Ropars, V., Charbonnier, J.-B., Miron, S., Craescu, C. T., Callebaut, I., Gilquin, B., Zinn-Justin, S. (2007). The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA. Nucleic Acids Res 35: 5898-5912 [Abstract] [Full Text]  


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