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Molecular and Cellular Biology, December 2006, p. 9387-9401, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01335-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Smc5/6 Is Required for Repair at Collapsed Replication Forks{triangledown}

Eleni Ampatzidou,1 Anja Irmisch,1 Matthew J. O'Connell,2 and Johanne M. Murray1*

Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom,1 Department of Oncological Sciences, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1130, New York, New York 100292

Received 20 July 2006/ Returned for modification 23 August 2006/ Accepted 2 October 2006

In eukaryotes, three pairs of structural-maintenance-of-chromosome (SMC) proteins are found in conserved multisubunit protein complexes required for chromosomal organization. Cohesin, the Smc1/3 complex, mediates sister chromatid cohesion while two condensin complexes containing Smc2/4 facilitate chromosome condensation. Smc5/6 scaffolds an essential complex required for homologous recombination repair. We have examined the response of smc6 mutants to the inhibition of DNA replication. We define homologous recombination-dependent and -independent functions for Smc6 during replication inhibition and provide evidence for a Rad60-independent function within S phase, in addition to a Rad60-dependent function following S phase. Both genetic and physical data show that when forks collapse (i.e., are not stabilized by the Cds1Chk2 checkpoint), Smc6 is required for the effective repair of resulting lesions but not for the recruitment of recombination proteins. We further demonstrate that when the Rad60-dependent, post-S-phase Smc6 function is compromised, the resulting recombination-dependent DNA intermediates that accumulate following release from replication arrest are not recognized by the G2/M checkpoint.

* Corresponding author. Mailing address: Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom. Phone: 44 1273 877191. Fax: 44 1273 678121. E-mail: j.m.murray{at}sussex.ac.uk.

{triangledown} Published ahead of print on 9 October 2006.

Molecular and Cellular Biology, December 2006, p. 9387-9401, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01335-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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