This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ampatzidou, E.
Right arrow Articles by Murray, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ampatzidou, E.
Right arrow Articles by Murray, J. M.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*HYDROXYUREA

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2006, p. 9387-9401, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01335-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Smc5/6 Is Required for Repair at Collapsed Replication Forks{triangledown}

Eleni Ampatzidou,1 Anja Irmisch,1 Matthew J. O'Connell,2 and Johanne M. Murray1*

Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom,1 Department of Oncological Sciences, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1130, New York, New York 100292

Received 20 July 2006/ Returned for modification 23 August 2006/ Accepted 2 October 2006

In eukaryotes, three pairs of structural-maintenance-of-chromosome (SMC) proteins are found in conserved multisubunit protein complexes required for chromosomal organization. Cohesin, the Smc1/3 complex, mediates sister chromatid cohesion while two condensin complexes containing Smc2/4 facilitate chromosome condensation. Smc5/6 scaffolds an essential complex required for homologous recombination repair. We have examined the response of smc6 mutants to the inhibition of DNA replication. We define homologous recombination-dependent and -independent functions for Smc6 during replication inhibition and provide evidence for a Rad60-independent function within S phase, in addition to a Rad60-dependent function following S phase. Both genetic and physical data show that when forks collapse (i.e., are not stabilized by the Cds1Chk2 checkpoint), Smc6 is required for the effective repair of resulting lesions but not for the recruitment of recombination proteins. We further demonstrate that when the Rad60-dependent, post-S-phase Smc6 function is compromised, the resulting recombination-dependent DNA intermediates that accumulate following release from replication arrest are not recognized by the G2/M checkpoint.

* Corresponding author. Mailing address: Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom. Phone: 44 1273 877191. Fax: 44 1273 678121. E-mail: j.m.murray{at}sussex.ac.uk.

{triangledown} Published ahead of print on 9 October 2006.

Molecular and Cellular Biology, December 2006, p. 9387-9401, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01335-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miyabe, I., Morishita, T., Shinagawa, H., Carr, A. M. (2009). Schizosaccharomyces pombe Cds1Chk2 regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60. J. Cell Sci. 122: 3638-3643 [Abstract] [Full Text]  
  • Outwin, E. A., Irmisch, A., Murray, J. M., O'Connell, M. J. (2009). Smc5-Smc6-Dependent Removal of Cohesin from Mitotic Chromosomes. Mol. Cell. Biol. 29: 4363-4375 [Abstract] [Full Text]  
  • Sollier, J., Driscoll, R., Castellucci, F., Foiani, M., Jackson, S. P., Branzei, D. (2009). The Saccharomyces cerevisiae Esc2 and Smc5-6 Proteins Promote Sister Chromatid Junction-mediated Intra-S Repair. Mol. Biol. Cell 20: 1671-1682 [Abstract] [Full Text]  
  • Pebernard, S., Perry, J. J. P., Tainer, J. A., Boddy, M. N. (2008). Nse1 RING-like Domain Supports Functions of the Smc5-Smc6 Holocomplex in Genome Stability. Mol. Biol. Cell 19: 4099-4109 [Abstract] [Full Text]  
  • Bailis, J. M., Luche, D. D., Hunter, T., Forsburg, S. L. (2008). Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins To Promote S-Phase Genome Stability. Mol. Cell. Biol. 28: 1724-1738 [Abstract] [Full Text]  
  • Taylor, E. M., Copsey, A. C., Hudson, J. J. R., Vidot, S., Lehmann, A. R. (2008). Identification of the Proteins, Including MAGEG1, That Make Up the Human SMC5-6 Protein Complex. Mol. Cell. Biol. 28: 1197-1206 [Abstract] [Full Text]  
  • Lee, K. M., Nizza, S., Hayes, T., Bass, K. L., Irmisch, A., Murray, J. M., O'Connell, M. J. (2007). Brc1-Mediated Rescue of Smc5/6 Deficiency: Requirement for Multiple Nucleases and a Novel Rad18 Function. Genetics 175: 1585-1595 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»