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Molecular and Cellular Biology, December 2006, p. 9402-9412, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01318-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Competence Factor ßFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC Coactivator{triangledown}

Jinsong Zhu,{dagger} Li Chen,{ddagger} Guoqiang Sun,§ and Alexander S. Raikhel*

Department of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, California 92521

Received 18 July 2006/ Returned for modification 22 August 2006/ Accepted 21 September 2006

Hormones provide generalized signals that are interpreted in a specific spatial and temporal manner by a developing or reproducing multicellular organism. The ability to respond to hormones is determined by the competence of a cell or a tissue. The ßFtz-F1 orphan nuclear receptor acts as a competence factor for the steroid hormone 20-hydroxyecdysone (20E) in Drosophila melanogaster metamorphosis and mosquito reproduction. The molecular nature of the ßFtz-F1 action remains unclear. We report that the protein-protein interaction between ßFtz-F1 and a p160/SRC coactivator of the ecdysone receptor, FISC, is crucial for the stage-specific expression of the 20E effector genes during mosquito reproduction. This interaction dramatically increases recruitment of FISC to the functional ecdysone receptor in a 20E-dependent manner. The presence of ßFtz-F1 facilitates loading of FISC and the ecdysone receptor on the target promoters, leading to enhanced local histone H4 acetylation and robust activation of the target genes. Thus, our results reveal the molecular basis of competence for the stage-specific 20E response.

* Corresponding author. Mailing address: Department of Entomology, University of California, Riverside, CA 92521. Phone: (951) 827-2129. Fax: (951) 827-2130. E-mail: alexander.raikhel{at}ucr.edu.

{triangledown} Published ahead of print on 2 October 2006.

{dagger} Present address: Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061.

{ddagger} Present address: Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.

§ Present address: Neurosciences Division, Beckman Research Institute, City of Hope, Duarte, CA 91010.

Molecular and Cellular Biology, December 2006, p. 9402-9412, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01318-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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