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Molecular and Cellular Biology, December 2006, p. 9424-9429, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01654-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Conservative Inheritance of Newly Synthesized DNA in Double-Strand Break-Induced Gene Conversion{triangledown}

Grzegorz Ira,1,2 Dominik Satory,2,{dagger} and James E. Haber1*

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454,1 Baylor College of Medicine, Department of Molecular and Human Genetics, One Baylor Plaza, Houston, Texas 770302

Received 5 September 2006/ Returned for modification 11 September 2006/ Accepted 20 September 2006

To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created during gene conversion are found at the repaired locus, leaving the donor unchanged. These results support suggestions that mitotic DSBs are primarily repaired by a synthesis-dependent strand-annealing mechanism. We also show that the proportion of crossing-over associated with DSB-induced ectopic recombination is not affected by the presence of nonhomologous sequences at one or both ends of the DSB or the presence of additional sequences that must be copied from the donor.

* Corresponding author. Mailing address: Rosenstiel Center, 415 South Street, Mail Stop 029, Brandeis University, Waltham, MA 02453-2728. Phone: (781) 736-2462. Fax: (781) 736-2405. E-mail: haber{at}brandeis.edu.

{triangledown} Published ahead of print on 9 October 2006.

{dagger} Permanent address: Nicholas Copernicus University, Torun, Poland.

Molecular and Cellular Biology, December 2006, p. 9424-9429, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01654-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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