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Molecular and Cellular Biology, December 2006, p. 9430-9441, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01656-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evidence that the Transcription Elongation Function of Rpb9 Is Involved in Transcription-Coupled DNA Repair in Saccharomyces cerevisiae{triangledown}

Shisheng Li,* Baojin Ding, Runqiang Chen, Christine Ruggiero, and Xuefeng Chen

Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803

Received 5 September 2006/ Returned for modification 25 September 2006/ Accepted 2 October 2006

Rpb9, a small nonessential subunit of RNA polymerase II, has been shown to have multiple transcription-related functions in Saccharomyces cerevisiae. These functions include promoting transcription elongation and mediating a subpathway of transcription-coupled repair (TCR) that is independent of Rad26, the homologue of human Cockayne syndrome complementation group B protein. Rpb9 is composed of three distinct domains: the N-terminal Zn1, the C-terminal Zn2, and the central linker. Here we show that the Zn1 and linker domains are essential, whereas the Zn2 domain is almost dispensable, for both transcription elongation and TCR functions. Impairment of transcription elongation, which does not dramatically compromise Rad26-mediated TCR, completely abolishes Rpb9-mediated TCR. Furthermore, Rpb9 appears to be dispensable for TCR if its transcription elongation function is compensated for by removing a transcription repression/elongation factor. Our data suggest that the transcription elongation function of Rpb9 is involved in TCR.

* Corresponding author. Mailing address: Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803. Phone: (225) 578-9102. Fax: (225) 578-9895. E-mail: shli{at}lsu.edu.

{triangledown} Published ahead of print on 9 October 2006.

Molecular and Cellular Biology, December 2006, p. 9430-9441, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01656-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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