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Molecular and Cellular Biology, February 2006, p. 852-862, Vol. 26, No. 3
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.3.852-862.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cell Cycle Requirements in Assembling Silent Chromatin in Saccharomyces cerevisiae

Ann L. Kirchmaier1* and Jasper Rine2

Department of Biochemistry and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907,1 Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-32022

Received 23 August 2005/ Returned for modification 13 October 2005/ Accepted 10 November 2005

The establishment of silencing at the silent mating-type locus, HMR, in Saccharomyces cerevisiae requires that yeast pass through S phase of the cell cycle, yet requires neither the initiation of DNA replication at the locus destined to become silenced nor the passage of a replication fork through that locus. We tested whether this S-phase requirement reflects a window within the cell cycle permissive for recruitment of Sir proteins to HMR. The S-phase-restricted event necessary for silencing occurred after recruitment of Sir proteins to HMR. Moreover, cells arrested in early S phase formed silent chromatin at HMR, provided HMR was on a nonreplicating template. Replicating templates required a later step for silencing. These results provide temporal resolution of discrete steps in the formation of silent chromatin and suggest that more than one cell cycle-regulated event may be necessary for the establishment of silencing.

* Corresponding author. Mailing address: Department of Biochemistry and Purdue Cancer Center, Purdue University, 175 S. University St., West Lafayette, IN 47907. Phone: (765) 494-0977. Fax: (765) 494-7897. E-mail: kirchmaier{at}purdue.edu.

Molecular and Cellular Biology, February 2006, p. 852-862, Vol. 26, No. 3
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.3.852-862.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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