This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boulard, M.
Right arrow Articles by Dimitrov, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boulard, M.
Right arrow Articles by Dimitrov, S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2006, p. 1518-1526, Vol. 26, No. 4
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.4.1518-1526.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The NH2 Tail of the Novel Histone Variant H2BFWT Exhibits Properties Distinct from Conventional H2B with Respect to the Assembly of Mitotic Chromosomes

Mathieu Boulard,1,2 Thierry Gautier,1 Gaelh Ouengue Mbele,1 Véronique Gerson,1 Ali Hamiche,3 Dimitar Angelov,2,4 Philippe Bouvet,2,4* and Stefan Dimitrov1,2*

Institut Albert Bonniot, INSERM U309, 38706 La Tronche Cedex, France,1 Ecole Normale Supérieure de Lyon, Laboratoire Joliot Curie, 46 Allée d'Italie, 69007 Lyon, France,2 Institut Andre Lwoff, CNRS UPR 9079, 7 rue Guy Moquet, 94800 Villejuif, France,3 Ecole Normale Supérieure de Lyon, LBMC, CNRS-UMR 5161, 46 Allée d'Italie, 69007 Lyon, France4

Received 12 September 2005/ Returned for modification 14 October 2005/ Accepted 21 November 2005

We have studied the functional and structural properties of nucleosomes reconstituted with H2BFWT, a recently identified putative histone variant of the H2B family with totally unknown function. We show that H2BFWT can replace the conventional histone H2B in the nucleosome. The presence of H2BFWT did not affect the overall structure of the nucleosome, and the H2BFWT nucleosomes exhibited the same stability as conventional nucleosomes. SWI/SNF was able to efficiently remodel and mobilize the H2BFWT nucleosomes. Importantly, H2BFWT, in contrast to conventional H2B, was unable to recruit chromosome condensation factors and to participate in the assembly of mitotic chromosomes. This was determined by the highly divergent (compared to conventional H2B) NH2 tail of H2BFWT. These data, in combination with the observations that H2BFWT was found by others in the sperm nuclei and appeared to be associated with the telomeric chromatin, suggest that H2BFWT could act as a specific epigenetic marker.

* Corresponding author. Mailing address for Stefan Dimitrov: Institut Albert Bonniot, INSERM U309, 38706 La Tronche Cedex, France. Phone: (33) 4 76 54 94 73. Fax: (33) 4 76 54 95 95. E-mail: stefan.dimitrov{at}ujf-grenoble.fr. Mailing address for Philippe Bouvet: Ecole Normale Supérieure de Lyon, Laboratoire Joliot Curie, 46 Allée d'Italie, 69007 Lyon, France. Phone and fax: (33) 4 72 72 80 16. E-mail: pbouvet{at}ens-lyon.fr.

Molecular and Cellular Biology, February 2006, p. 1518-1526, Vol. 26, No. 4
0022-538X/06/$08.00+0     doi:10.1128/MCB.26.4.1518-1526.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stouffs, K., Tournaye, H., Liebaers, I., Lissens, W. (2009). Male infertility and the involvement of the X chromosome. Hum Reprod Update 15: 623-637 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»