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Molecular and Cellular Biology, March 2006, p. 1579-1588, Vol. 26, No. 5
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.5.1579-1588.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Unique Role for the UbL-UbA Protein Ddi1 in Turnover of SCF^Ufo1 Complexes

Yelena Ivantsiv, Ludmila Kaplun, Regina Tzirkin-Goldin, Nitzan Shabek, and Dina Raveh^*

Department of Life Sciences, Ben Gurion University of the Negev, Beersheba 84105, Israel

Received 7 June 2005/ Returned for modification 10 July 2005/ Accepted 18 November 2005

SCF complexes are E3 ubiquitin-protein ligases that mediate degradation of regulatory and signaling proteins and control G₁/S cell cycle progression by degradation of G₁ cyclins and the cyclin-dependent kinase inhibitor, Sic1. Interchangeable F-box proteins bind the core SCF components; each recruits a specific subset of substrates for ubiquitylation. The F-box proteins themselves are rapidly turned over by autoubiquitylation, allowing rapid recycling of SCF complexes. Here we report a role for the UbL-UbA protein Ddi1 in the turnover of the F-box protein, Ufo1. Ufo1 is unique among F-box proteins in having a domain comprising multiple ubiquitin-interacting motifs (UIMs) that mediate its turnover. Deleting the UIMs leads to stabilization of Ufo1 and to cell cycle arrest at G₁/S of cells with long buds resembling skp1 mutants. Cells accumulate substrates of other F-box proteins, indicating that the SCF pathway of substrate ubiquitylation is inhibited. Ufo1 interacts with Ddi1 via its UIMs, and ddi1 cells arrest when full-length UFO1 is overexpressed. These results imply a role for the UIMs in turnover of SCF^Ufo1 complexes that is dependent on Ddi1, a novel activity for an UbL-UbA protein.

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* Corresponding author. Mailing address: Department of Life Sciences, Ben Gurion University of the Negev, Beersheba 84105, Israel. Phone: 972-8-646-1371. Fax: 972-8-647-9190. E-mail: raveh{at}bgu.ac.il.

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Molecular and Cellular Biology, March 2006, p. 1579-1588, Vol. 26, No. 5
0022-538X/06/$08.00+0     doi:10.1128/MCB.26.5.1579-1588.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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