This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Win, T. Z. Articles by Wang, S.-W. Search for Related Content PubMed PubMed Citation Articles by Win, T. Z. Articles by Wang, S.-W.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2006, p. 1710-1721, Vol. 26, No. 5
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.5.1710-1721.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Requirement of Fission Yeast Cid14 in Polyadenylation of rRNAs

Thein Z. Win,¹ Simon Draper,^2, Rebecca L. Read,² James Pearce,¹ Chris J. Norbury,³ and Shao-Win Wang^1*

Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom,¹ Cancer Research UK Laboratories, University of Oxford, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom,² Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom³

Received 25 October 2005/ Returned for modification 18 November 2005/ Accepted 14 December 2005

Polyadenylation in eukaryotes is conventionally associated with increased nuclear export, translation, and stability of mRNAs. In contrast, recent studies suggest that the Trf4 and Trf5 proteins, members of a widespread family of noncanonical poly(A) polymerases, share an essential function in Saccharomyces cerevisiae that involves polyadenylation of nuclear RNAs as part of a pathway of exosome-mediated RNA turnover. Substrates for this pathway include aberrantly modified tRNAs and precursors of snoRNAs and rRNAs. Here we show that Cid14 is a Trf4/5 functional homolog in the distantly related fission yeast Schizosaccharomyces pombe. Unlike trf4 trf5 double mutants, cells lacking Cid14 are viable, though they suffer an increased frequency of chromosome missegregation. The Cid14 protein is constitutively nucleolar and is required for normal nucleolar structure. A minor population of polyadenylated rRNAs was identified. These RNAs accumulated in an exosome mutant, and their presence was largely dependent on Cid14, in line with a role for Cid14 in rRNA degradation. Surprisingly, both fully processed 25S rRNA and rRNA processing intermediates appear to be channeled into this pathway. Our data suggest that additional substrates may include the mRNAs of genes involved in meiotic regulation. Polyadenylation-assisted nuclear RNA turnover is therefore likely to be a common eukaryotic mechanism affecting diverse biological processes.

Present address: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.

This article has been cited by other articles:

• Wang, S.-W., Stevenson, A. L., Kearsey, S. E., Watt, S., Bahler, J. (2008). Global Role for Polyadenylation-Assisted Nuclear RNA Degradation in Posttranscriptional Gene Silencing. Mol. Cell. Biol. 28: 656-665 [Abstract] [Full Text]  
• Cristodero, M., Clayton, C. E. (2007). Trypanosome MTR4 is involved in rRNA processing. Nucleic Acids Res 35: 7023-7030 [Abstract] [Full Text]  
• Martin, G., Keller, W. (2007). RNA-specific ribonucleotidyl transferases. RNA 13: 1834-1849 [Abstract] [Full Text]  
• Rissland, O. S., Mikulasova, A., Norbury, C. J. (2007). Efficient RNA Polyuridylation by Noncanonical Poly(A) Polymerases. Mol. Cell. Biol. 27: 3612-3624 [Abstract] [Full Text]  
• Schilders, G., van Dijk, E., Pruijn, G. J.M. (2007). C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing. Nucleic Acids Res 0: gkm082v1-9 [Abstract] [Full Text]  
• Reis, C. C., Campbell, J. L. (2007). Contribution of Trf4/5 and the Nuclear Exosome to Genome Stability Through Regulation of Histone mRNA Levels in Saccharomyces cerevisiae. Genetics 175: 993-1010 [Abstract] [Full Text]  
• Hinas, A., Larsson, P., Avesson, L., Kirsebom, L. A., Virtanen, A., Soderbom, F. (2006). Identification of the Major Spliceosomal RNAs in Dictyostelium discoideum Reveals Developmentally Regulated U2 Variants and Polyadenylated snRNAs. Eukaryot Cell 5: 924-934 [Abstract] [Full Text]