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Molecular and Cellular Biology, March 2006, p. 1817-1825, Vol. 26, No. 5
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.5.1817-1825.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

{gamma}-Parvin Is Dispensable for Hematopoiesis, Leukocyte Trafficking, and T-Cell-Dependent Antibody Response{dagger}

Haiyan Chu,1 Ingo Thievessen,1 Michael Sixt,1 Tim Lämmermann,1 Ari Waisman,2,{ddagger} Attila Braun,1 Angelika A. Noegel,3 and Reinhard Fässler1*

Department of Molecular Medicine, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany,1 Laboratory of Molecular Immunology, Institute for Genetics, University of Cologne, Zülpicher Str. 47, 50674 Cologne, Germany,2 Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931 Cologne, Germany3

Received 12 August 2005/ Returned for modification 21 September 2005/ Accepted 30 November 2005

Integrins regulate cell behavior through the assembly of multiprotein complexes at the site of cell adhesion. Parvins are components of such a multiprotein complex. They consist of three members ({alpha}-, ß-, and {gamma}-parvin), form a functional complex with integrin-linked kinase (ILK) and PINCH, and link integrins to the actin cytoskeleton. Whereas {alpha}- and ß-parvins are widely expressed, {gamma}-parvin has been reported to be expressed in hematopoietic organs. In the present study, we report the expression pattern of the parvins in hematopoietic cells and the phenotypic analysis of {gamma}-parvin-deficient mice. Whereas {alpha}-parvin is not expressed in hematopoietic cells, ß-parvin is only found in myeloid cells and {gamma}-parvin is present in both cells of the myeloid and lymphoid lineages, where it binds ILK. Surprisingly, loss of {gamma}-parvin expression had no effect on blood cell differentiation, proliferation, and survival and no consequence for the T-cell-dependent antibody response and lymphocyte and dendritic cell migration. These data indicate that despite the high expression of {gamma}-parvin in hematopoietic cells it must play a more subtle role for blood cell homeostasis.

* Corresponding author. Mailing address: Department of Molecular Medicine, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. Phone: (49) 89-8578-2424. Fax: (49) 89-8578-2422. E-mail: faessler{at}biochem.mpg.de.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Present address: Medizinsche Klinik und Poliklinik, Johannes Gutenberg-Universitat Mainz, 55131 Mainz, Germany.

Molecular and Cellular Biology, March 2006, p. 1817-1825, Vol. 26, No. 5
0022-538X/06/$08.00+0     doi:10.1128/MCB.26.5.1817-1825.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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