This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Binda, O.
Right arrow Articles by Branton, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Binda, O.
Right arrow Articles by Branton, P. E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2006, p. 1917-1931, Vol. 26, No. 5
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.5.1917-1931.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence{dagger}

Olivier Binda,1 Jean-Sébastien Roy,1 and Philip E. Branton1,2,3*

Department of Biochemistry,1 Department of Oncology,2 McGill Cancer Center, McGill University, McIntyre Medical Building, 3655 Promenade Sir William Osler, Montréal, Québec H3G 1Y6, Canada3

Received 29 June 2005/ Returned for modification 23 August 2005/ Accepted 5 December 2005

The retinoblastoma binding protein 1 (RBP1) appears to be an important factor in the repression of E2F-dependent transcription by the retinoblastoma protein (pRB) family. The recent identification of the breast carcinoma associated antigen (BCAA) as an RBP1-like protein led us to investigate its biological properties and compare them to RBP1. Like RBP1, BCAA contains a carboxy-terminal R2 domain that elicits histone deacetylase (HDAC)-dependent transcriptional repression via interactions with the SAP30 subunit of the Sin3/HDAC complex. Each RBP1 family member also contains two HDAC-independent repression activities within a region termed R1, which can be subdivided into a SUMOylated moiety (R1{sigma}) and a predicted {alpha}-helical region (R1{alpha}). R1{alpha} is embedded within the ARID region and represses basal transcription only, whereas R1{sigma} represses both basal and activated transcription and depends on SUMOylation. Overexpression of either RBP1 or BCAA, but not the truncated BCAAMCF-7 isoform that is overexpressed in breast cancer cells, caused a profound inhibition of cell proliferation and induced expression of a senescence marker. In each case the presence of both R1 and R2 was necessary for suppression of cell growth, suggesting that both R1 and R2 transcriptional repression activities play a role in RBP1 family protein-mediated regulation of cellular proliferation.

* Corresponding author. Mailing address: Department of Biochemistry, McGill University, McIntyre Medical Bldg., Rm. 702, 3655 Promenade Sir William Osler, Montréal, Québec H3G 1Y6, Canada. Phone: (514) 398-8350. Fax: (514) 398-8845. E-mail: philip.branton{at}mcgill.ca.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, March 2006, p. 1917-1931, Vol. 26, No. 5
0022-538X/06/$08.00+0     doi:10.1128/MCB.26.5.1917-1931.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • He, Y., Imhoff, R., Sahu, A., Radhakrishnan, I. (2009). Solution structure of a novel zinc finger motif in the SAP30 polypeptide of the Sin3 corepressor complex and its potential role in nucleic acid recognition. Nucleic Acids Res 37: 2142-2152 [Abstract] [Full Text]  
  • Wu, M.-Y., Eldin, K. W., Beaudet, A. L. (2008). Identification of Chromatin Remodeling Genes Arid4a and Arid4b as Leukemia Suppressor Genes. JNCI J Natl Cancer Inst 100: 1247-1259 [Abstract] [Full Text]  
  • Hurst, D. R., Xie, Y., Vaidya, K. S., Mehta, A., Moore, B. P., Accavitti-Loper, M. A., Samant, R. S., Saxena, R., Silveira, A. C., Welch, D. R. (2008). Alterations of BRMS1-ARID4A Interaction Modify Gene Expression but Still Suppress Metastasis in Human Breast Cancer Cells. J. Biol. Chem. 283: 7438-7444 [Abstract] [Full Text]  
  • Landais, S., Landry, S., Legault, P., Rassart, E. (2007). Oncogenic Potential of the miR-106-363 Cluster and Its Implication in Human T-Cell Leukemia. Cancer Res. 67: 5699-5707 [Abstract] [Full Text]  
  • Mascle, X. H., Germain-Desprez, D., Huynh, P., Estephan, P., Aubry, M. (2007). Sumoylation of the Transcriptional Intermediary Factor 1beta (TIF1beta), the Co-repressor of the KRAB Multifinger Proteins, Is Required for Its Transcriptional Activity and Is Modulated by the KRAB Domain. J. Biol. Chem. 282: 10190-10202 [Abstract] [Full Text]  
  • Monroe, D. G., Secreto, F. J., Hawse, J. R., Subramaniam, M., Khosla, S., Spelsberg, T. C. (2006). Estrogen Receptor Isoform-specific Regulation of the Retinoblastoma-binding Protein 1 (RBBP1) Gene: ROLES OF AF1 AND ENHANCER ELEMENTS. J. Biol. Chem. 281: 28596-28604 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»