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Molecular and Cellular Biology, April 2006, p. 3085-3097, Vol. 26, No. 8
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.8.3085-3097.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evidence that Poly(A) Binding Protein C1 Binds Nuclear Pre-mRNA Poly(A) Tails

Nao Hosoda, Fabrice Lejeune,{dagger} and Lynne E. Maquat*

Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Ave., Box 712, Rochester, New York 14642

Received 23 August 2005/ Returned for modification 20 January 2006/ Accepted 24 January 2006

In mammalian cells, poly(A) binding protein C1 (PABP C1) has well-known roles in mRNA translation and decay in the cytoplasm. However, PABPC1 also shuttles in and out of the nucleus, and its nuclear function is unknown. Here, we show that PABPC1, like the major nuclear poly(A) binding protein PABPN1, associates with nuclear pre-mRNAs that are polyadenylated and intron containing. PABPC1 does not bind nonpolyadenylated histone mRNA, indicating that the interaction of PABPC1 with pre-mRNA requires a poly(A) tail. Consistent with this conclusion, UV cross-linking results obtained using intact cells reveal that PABPC1 binds directly to pre-mRNA poly(A) tails in vivo. We also show that PABPC1 immunopurifies with poly(A) polymerase, suggesting that PABPC1 is acquired by polyadenylated transcripts during poly(A) tail synthesis. Our findings demonstrate that PABPC1 associates with polyadenylated transcripts earlier in mammalian mRNA biogenesis than previously thought and offer insights into the mechanism by which PABPC1 is recruited to newly synthesized poly(A). Our results are discussed in the context of pre-mRNA processing and stability and mRNA trafficking and the pioneer round of translation.

* Corresponding author. Mailing address: Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Ave., Box 712, Rochester, NY 14642. Phone: (585) 273-5640. Fax: (585) 271-2683. E-mail: lynne_maquat{at}urmc.rochester.edu.

{dagger} Present address: Institut de Génétique Moléculaire de Montpellier, CNRS, Montpellier, France.

Molecular and Cellular Biology, April 2006, p. 3085-3097, Vol. 26, No. 8
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.8.3085-3097.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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