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Molecular and Cellular Biology, January 2007, p. 253-266, Vol. 27, No. 1
0270-7306/07/$08.00+0     doi:10.1128/MCB.01071-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Dynamic Interplay of Transcriptional Machinery and Chromatin Regulates "Late" Expression of the Chemokine RANTES in T Lymphocytes{triangledown}

Yong-Tae Ahn, Boli Huang, Lisa McPherson, Carol Clayberger, and Alan M. Krensky*

Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, Stanford, California

Received 14 June 2006/ Returned for modification 21 July 2006/ Accepted 16 October 2006

The chemokine RANTES (regulated upon activation normal T cell expressed and secreted) is expressed "late" (3 to 5 days) after activation in T lymphocytes. In order to understand the molecular events that accompany changes in gene expression, a detailed analysis of the interplay between transcriptional machinery and chromatin on the RANTES promoter over time was undertaken. Krüppel-like factor 13 (KLF13), a sequence-specific DNA binding transcription factor, orchestrates the induction of RANTES expression in T lymphocytes by ordered recruitment of effector molecules, including Nemo-like kinase, p300/cyclic AMP response element binding protein (CBP), p300/CBP-associated factor, and Brahma-related gene 1, that initiate sequential changes in phosphorylation and acetylation of histones and ATP-dependent chromatin remodeling near the TATA box of the RANTES promoter. These events recruit RNA polymerase II to the RANTES promoter and are responsible for late expression of RANTES in T lymphocytes. Therefore, KLF13 is a key regulator of late RANTES expression in T lymphocytes.

* Corresponding author. Mailing address: Division of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University School of Medicine, CCSR Room 2105, 300 Pasteur Drive, Stanford, CA 94305-5164. Phone: (650) 498-6073. Fax: (650) 498-6077. E-mail: Krensky{at}stanford.edu.

{triangledown} Published ahead of print on 30 October 2006.

Molecular and Cellular Biology, January 2007, p. 253-266, Vol. 27, No. 1
0270-7306/07/$08.00+0     doi:10.1128/MCB.01071-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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