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Molecular and Cellular Biology, May 2007, p. 3780-3792, Vol. 27, No. 10
0270-7306/07/$08.00+0     doi:10.1128/MCB.01761-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

PIASy-Mediated Sumoylation of Yin Yang 1 Depends on Their Interaction but Not the RING Finger{triangledown} ,{dagger}

Zhiyong Deng, Meimei Wan, and Guangchao Sui*

Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157

Received 18 September 2006/ Returned for modification 13 November 2006/ Accepted 2 March 2007

As a multifunctional protein, Yin Yang 1 (YY1) has been demonstrated to regulate both gene expression and protein posttranslational modifications. However, gaps still exist in our knowledge of how YY1 can be modified and what the consequences of its modifications are. Here we report that YY1 protein can be sumoylated both in vivo and in vitro. We have identified lysine 288 as the major sumoylation site of YY1. We also discovered that PIASy, a SUMO E3 ligase, is a novel YY1-interacting protein and can stimulate the sumoylation of YY1 both in vitro and in vivo. Importantly, the effects of PIASy mutants on in vivo YY1 sumoylation correlate with the YY1-PIASy interaction but do not depend on the RING finger domain of PIASy. This regulation is unique to YY1 sumoylation because PIASy-mediated p53 sumoylation still relies on the integrity of PIASy, which is also true of all of the previously identified substrates of PIASy. In addition, PIASy colocalizes with YY1 in the nucleus, stabilizes YY1 in vivo, and differentially regulates YY1 transcriptional activity on different target promoters. This study demonstrates that YY1 is a target of SUMOs and reveals a novel feature of a SUMO E3 ligase in the PIAS family that selectively stimulates protein sumoylation independent of the RING finger domain.

* Corresponding author. Mailing address: Department of Cancer Biology, Wake Forest University School of Medicine, Hanes 4052, Medical Center Boulevard, Winston-Salem, NC 27157. Phone: (336) 713-0052. Fax: (336) 716-0255. E-mail: gsui{at}wfubmc.edu

{triangledown} Published ahead of print on 12 March 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, May 2007, p. 3780-3792, Vol. 27, No. 10
0270-7306/07/$08.00+0     doi:10.1128/MCB.01761-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Palmer, M. B., Majumder, P., Cooper, J. C., Yoon, H., Wade, P. A., Boss, J. M. (2009). Yin Yang 1 Regulates the Expression of Snail through a Distal Enhancer. Mol Cancer Res 7: 221-229 [Abstract] [Full Text]  


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