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Molecular and Cellular Biology, June 2007, p. 4157-4165, Vol. 27, No. 11
0270-7306/07/$08.00+0     doi:10.1128/MCB.00120-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Depletion of the Yeast Nuclear Exosome Subunit Rrp6 Results in Accumulation of Polyadenylated RNAs in a Discrete Domain within the Nucleolus{triangledown}

Tiago Carneiro,1 Célia Carvalho,1 José Braga,1 José Rino,1 Laura Milligan,2 David Tollervey,2 and Maria Carmo-Fonseca1*

Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal,1 Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom2

Received 19 January 2007/ Returned for modification 14 February 2007/ Accepted 20 March 2007

Recent data reveal that a substantial fraction of transcripts generated by RNA polymerases I, II, and III are rapidly degraded in the nucleus by the combined action of the exosome and a noncanonical poly(A) polymerase activity. This work identifies a domain within the yeast nucleolus that is enriched in polyadenylated RNAs in the absence of the nuclear exosome RNase Rrp6 or the exosome cofactor Mtr4. In normal yeast cells, poly(A)+ RNA was undetectable in the nucleolus but the depletion of either Rrp6 or Mtr4 led to the accumulation of polyadenylated RNAs in a discrete subnucleolar region. This nucleolar poly(A) domain is enriched for the U14 snoRNA and the snoRNP protein Nop1 but is distinct from the nucleolar body that functions in snoRNA maturation. In strains lacking both Rrp6 and the poly(A) polymerase Trf4, the accumulation of poly(A)+ RNA was suppressed, suggesting the involvement of the Trf4-Air1/2-Mtr4 polyadenylation (TRAMP) complex. The accumulation of polyadenylated snoRNAs in a discrete nucleolar domain may promote their recognition as substrates for the exosome.

* Corresponding author. Mailing address: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal. Phone: 351 21 7940157. Fax: 351 21 79 99 412. E-mail: carmo.fonseca{at}fm.ul.pt

{triangledown} Published ahead of print on 2 April 2007.

Molecular and Cellular Biology, June 2007, p. 4157-4165, Vol. 27, No. 11
0270-7306/07/$08.00+0     doi:10.1128/MCB.00120-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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