This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Senese, S.
Right arrow Articles by Chiocca, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Senese, S.
Right arrow Articles by Chiocca, S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2007, p. 4784-4795, Vol. 27, No. 13
0270-7306/07/$08.00+0     doi:10.1128/MCB.00494-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role for Histone Deacetylase 1 in Human Tumor Cell Proliferation{triangledown}

Silvia Senese,1,2 Katrin Zaragoza,1 Simone Minardi,2 Ivan Muradore,1 Simona Ronzoni,1 Alfonso Passafaro,1 Loris Bernard,2 Giulio F. Draetta,3 Myriam Alcalay,2 Christian Seiser,4 and Susanna Chiocca1,2*

European Institute of Oncology, Department of Experimental Oncology, 20141 Milan, Italy,1 IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy,2 Cancer Research, Merck Research Laboratories, Basic Research, 33 Avenue Louis Pasteur, Boston, Massachusetts 02115,3 Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Dr. Bohr-Gasse 9/2, A-1030 Vienna, Austria4

Received 21 March 2007/ Returned for modification 11 April 2007/ Accepted 18 April 2007

Posttranslational modifications of core histones are central to the regulation of gene expression. Histone deacetylases (HDACs) repress transcription by deacetylating histones, and class I HDACs have a crucial role in mouse, Xenopus laevis, zebra fish, and Caenorhabditis elegans development. The role of individual class I HDACs in tumor cell proliferation was investigated using RNA interference-mediated protein knockdown. We show here that in the absence of HDAC1 cells can arrest either at the G1 phase of the cell cycle or at the G2/M transition, resulting in the loss of mitotic cells, cell growth inhibition, and an increase in the percentage of apoptotic cells. On the contrary, HDAC2 knockdown showed no effect on cell proliferation unless we concurrently knocked down HDAC1. Using gene expression profiling analysis, we found that inactivation of HDAC1 affected the transcription of specific target genes involved in proliferation and apoptosis. Furthermore, HDAC2 downregulation did not cause significant changes compared to control cells, while inactivation of HDAC1, HDAC1 plus HDAC2, or HDAC3 resulted in more distinct clusters. Loss of these HDACs might impair cell cycle progression by affecting not only the transcription of specific target genes but also other biological processes. Our data support the idea that a drug targeting specific HDACs could be highly beneficial in the treatment of cancer.

* Corresponding author. Mailing address: European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, 20141 Milan, Italy. Phone: 39-02-57489835. Fax: 39-02-57489851. E-mail: susanna.chiocca{at}ifom-ieo-campus.it

{triangledown} Published ahead of print on 30 April 2007.

Molecular and Cellular Biology, July 2007, p. 4784-4795, Vol. 27, No. 13
0270-7306/07/$08.00+0     doi:10.1128/MCB.00494-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schrump, D. S. (2009). Cytotoxicity Mediated by Histone Deacetylase Inhibitors in Cancer Cells: Mechanisms and Potential Clinical Implications. Clin. Cancer Res. 15: 3947-3957 [Abstract] [Full Text]  
  • Haberland, M., Johnson, A., Mokalled, M. H., Montgomery, R. L., Olson, E. N. (2009). Genetic dissection of histone deacetylase requirement in tumor cells. Proc. Natl. Acad. Sci. USA 106: 7751-7755 [Abstract] [Full Text]  
  • Duong, V., Bret, C., Altucci, L., Mai, A., Duraffourd, C., Loubersac, J., Harmand, P.-O., Bonnet, S., Valente, S., Maudelonde, T., Cavailles, V., Boulle, N. (2008). Specific Activity of Class II Histone Deacetylases in Human Breast Cancer Cells. Mol Cancer Res 6: 1908-1919 [Abstract] [Full Text]  
  • Noh, S. J., Jeong, W. J., Rho, J. H., Shin, D. M., Ahn, H. B., Park, W. C., Rho, S. H., Soung, Y. H., Kim, T. H., Park, B. S., Yoo, Y. H. (2008). Sensitization of RPE Cells by {alpha}B-Crystallin siRNA to SAHA-Induced Stage 1 Apoptosis through Abolishing the Association of {alpha}B-Crystallin with HDAC1 in SC35 Speckles. IOVS 49: 4753-4759 [Abstract] [Full Text]  
  • Li, P., Yao, H., Zhang, Z., Li, M., Luo, Y., Thompson, P. R., Gilmour, D. S., Wang, Y. (2008). Regulation of p53 Target Gene Expression by Peptidylarginine Deiminase 4. Mol. Cell. Biol. 28: 4745-4758 [Abstract] [Full Text]  
  • Su, H., Altucci, L., You, Q. (2008). Competitive or noncompetitive, that's the question: research toward histone deacetylase inhibitors. Molecular Cancer Therapeutics 7: 1007-1012 [Abstract] [Full Text]  
  • Fournel, M., Bonfils, C., Hou, Y., Yan, P. T., Trachy-Bourget, M.-C., Kalita, A., Liu, J., Lu, A.-H., Zhou, N. Z., Robert, M.-F., Gillespie, J., Wang, J. J., Ste-Croix, H., Rahil, J., Lefebvre, S., Moradei, O., Delorme, D., MacLeod, A. R., Besterman, J. M., Li, Z. (2008). MGCD0103, a novel isotype-selective histone deacetylase inhibitor, has broad spectrum antitumor activity in vitro and in vivo. Molecular Cancer Therapeutics 7: 759-768 [Abstract] [Full Text]  
  • Lin, W., Hashimoto, S.-i., Seo, H., Shibata, T., Ohta, K. (2008). Modulation of immunoglobulin gene conversion frequency and distribution by the histone deacetylase HDAC2 in chicken DT40.. GENES CELLS 13: 255-268 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»