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Molecular and Cellular Biology, August 2007, p. 5639-5649, Vol. 27, No. 16
0270-7306/07/$08.00+0     doi:10.1128/MCB.00418-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Regulation of Telomere Structure and Functions by Subunits of the INO80 Chromatin Remodeling Complex ^,

Eun Young Yu,¹ Olga Steinberg-Neifach,^1, Alain T. Dandjinou,¹ Frances Kang,¹ Ashby J. Morrison,² Xuetong Shen,² and Neal F. Lue^1*

Department of Microbiology and Immunology, W. R. Hearst Microbiology Research Center, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021,¹ Department of Carcinogenesis, M. D. Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957²

Received 9 March 2007/ Returned for modification 23 April 2007/ Accepted 1 June 2007

ATP-dependent chromatin remodeling complexes have been implicated in the regulation of transcription, replication, and more recently DNA double-strand break repair. Here we report that the Ies3p subunit of the Saccharomyces cerevisiae INO80 chromatin remodeling complex interacts with a conserved tetratricopeptide repeat domain of the telomerase protein Est1p. Deletion of IES3 and some other subunits of the complex induced telomere elongation and altered telomere position effect. In telomerase-negative mutants, loss of Ies3p delayed the emergence of recombinational survivors and stimulated the formation of extrachromosomal telomeric circles in survivors. Deletion of IES3 also resulted in heightened levels of telomere-telomere fusions in telomerase-deficient strains. In addition, a delay in survivor formation was observed in an Arp8p-deficient mutant. Because Arp8p is required for the chromatin remodeling activity of the INO80 complex, the complex may promote recombinational telomere maintenance by altering chromatin structure. Consistent with this notion, we observed preferential localization of multiple subunits of the INO80 complex to telomeres. Our results reveal novel functions for a subunit of the telomerase complex and the INO80 chromatin remodeling complex.

Published ahead of print on 11 June 2007.

Supplemental material for this article may be found at http://mcb.asm.org/.

Present address: Eugenio Maria de Hustes Community College, 500 Grand Concourse, Bronx, NY 10451.

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