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Molecular and Cellular Biology, September 2007, p. 6520-6531, Vol. 27, No. 18
0270-7306/07/$08.00+0     doi:10.1128/MCB.00733-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Yeast Cap Binding Complex Impedes Recruitment of Cleavage Factor IA to Weak Termination Sites{triangledown}

Chi-Ming Wong, Hongfang Qiu, Cuihua Hu, Jinsheng Dong, and Alan G. Hinnebusch*

Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892

Received 26 April 2007/ Returned for modification 24 May 2007/ Accepted 10 July 2007

Nuclear cap binding complex (CBC) is recruited cotranscriptionally and stimulates spliceosome assembly on nascent mRNAs; however, its possible functions in regulating transcription elongation or termination were not well understood. We show that, while CBC appears to be dispensable for normal rates and processivity of elongation by RNA polymerase II (Pol II), it plays a direct role in preventing polyadenylation at weak termination sites. Similarly to Npl3p, with which it interacts, CBC suppresses the weak terminator of the gal10-{Delta}56 mutant allele by impeding recruitment of termination factors Pcf11p and Rna15p (subunits of cleavage factor IA [CF IA]) and does so without influencing Npl3p occupancy at the termination site. Importantly, deletion of CBC subunits or NPL3 also increases termination at a naturally occurring weak poly(A) site in the RNA14 coding sequences. We also show that CBC is most likely recruited directly to the cap of nascent transcripts rather than interacting first with transcriptional activators or the phosphorylated C-terminal domain of Pol II. Thus, our findings illuminate the mechanism of CBC recruitment and extend its function in Saccharomyces cerevisiae beyond mRNA splicing and degradation of aberrant nuclear mRNAs to include regulation of CF IA recruitment at poly(A) selection sites.

* Corresponding author. Mailing address: NIH, Building 6A/Room B1A-13, Bethesda, MD 20892. Phone: (301) 496-4480. Fax: (301) 496-6828. E-mail: ahinnebusch{at}nih.gov

{triangledown} Published ahead of print on 16 July 2007.

Molecular and Cellular Biology, September 2007, p. 6520-6531, Vol. 27, No. 18
0270-7306/07/$08.00+0     doi:10.1128/MCB.00733-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hossain, M. A., Claggett, J. M., Nguyen, T., Johnson, T. L. (2009). The cap binding complex influences H2B ubiquitination by facilitating splicing of the SUS1 pre-mRNA. RNA 15: 1515-1527 [Abstract] [Full Text]  
  • Lund, M. K., Kress, T. L., Guthrie, C. (2008). Autoregulation of Npl3, a Yeast SR Protein, Requires a Novel Downstream Region and Serine Phosphorylation. Mol. Cell. Biol. 28: 3873-3881 [Abstract] [Full Text]  


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