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Molecular and Cellular Biology, October 2007, p. 6639-6646, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00798-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Ribosomal Slowdown Mediates Translational Arrest during Cellular Division{triangledown}

Gilad Sivan,1 Nancy Kedersha,2 and Orna Elroy-Stein1*

Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel,1 Division of Rheumatology, Immunology and Allergy, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts2

Received 7 May 2007/ Returned for modification 4 June 2007/ Accepted 20 July 2007

Global mRNA translation is transiently inhibited during cellular division. We demonstrate that mitotic cells contain heavy polysomes, but these are significantly less translationally active than polysomes in cycling cells. Several observations indicate that mitotic translational attenuation occurs during the elongation stage: (i) in cycling nonsynchronized cultures, only mitotic cells fail to assemble stress granules when treated with agents that inhibit translational initiation; (ii) mitotic cells contain fewer free 80S complexes, which are less sensitive to high salt disassembly; (iii) mitotic polysomes are more resistant to enforced disassembly using puromycin; and (iv) ribosome transit time increases during mitosis. Elongation slowdown guarantees that polysomes are retained even if initiation is inhibited at the same time. Stalling translating ribosomes during mitosis may protect mRNAs and allow rapid resumption of translation immediately upon entry into the G1 phase.

* Corresponding author. Mailing address: Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. Phone: 972 3 640 9153. Fax: 972 3 642 2046. E-mail: ornaes{at}tauex.tau.ac.il

{triangledown} Published ahead of print on 30 July 2007.

Molecular and Cellular Biology, October 2007, p. 6639-6646, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00798-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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