This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sivan, G.
Right arrow Articles by Elroy-Stein, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sivan, G.
Right arrow Articles by Elroy-Stein, O.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2007, p. 6639-6646, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00798-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Ribosomal Slowdown Mediates Translational Arrest during Cellular Division{triangledown}

Gilad Sivan,1 Nancy Kedersha,2 and Orna Elroy-Stein1*

Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel,1 Division of Rheumatology, Immunology and Allergy, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts2

Received 7 May 2007/ Returned for modification 4 June 2007/ Accepted 20 July 2007

Global mRNA translation is transiently inhibited during cellular division. We demonstrate that mitotic cells contain heavy polysomes, but these are significantly less translationally active than polysomes in cycling cells. Several observations indicate that mitotic translational attenuation occurs during the elongation stage: (i) in cycling nonsynchronized cultures, only mitotic cells fail to assemble stress granules when treated with agents that inhibit translational initiation; (ii) mitotic cells contain fewer free 80S complexes, which are less sensitive to high salt disassembly; (iii) mitotic polysomes are more resistant to enforced disassembly using puromycin; and (iv) ribosome transit time increases during mitosis. Elongation slowdown guarantees that polysomes are retained even if initiation is inhibited at the same time. Stalling translating ribosomes during mitosis may protect mRNAs and allow rapid resumption of translation immediately upon entry into the G1 phase.

* Corresponding author. Mailing address: Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. Phone: 972 3 640 9153. Fax: 972 3 642 2046. E-mail: ornaes{at}tauex.tau.ac.il

{triangledown} Published ahead of print on 30 July 2007.

Molecular and Cellular Biology, October 2007, p. 6639-6646, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00798-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Raveh-Amit, H., Maissel, A., Poller, J., Marom, L., Elroy-Stein, O., Shapira, M., Livneh, E. (2009). Translational Control of Protein Kinase C{eta} by Two Upstream Open Reading Frames. Mol. Cell. Biol. 29: 6140-6148 [Abstract] [Full Text]  
  • Loschi, M., Leishman, C. C., Berardone, N., Boccaccio, G. L. (2009). Dynein and kinesin regulate stress-granule and P-body dynamics. J. Cell Sci. 122: 3973-3982 [Abstract] [Full Text]  
  • Delannoy, E., Le Ret, M., Faivre-Nitschke, E., Estavillo, G. M., Bergdoll, M., Taylor, N. L., Pogson, B. J., Small, I., Imbault, P., Gualberto, J. M. (2009). Arabidopsis tRNA Adenosine Deaminase Arginine Edits the Wobble Nucleotide of Chloroplast tRNAArg(ACG) and Is Essential for Efficient Chloroplast Translation. Plant Cell 21: 2058-2071 [Abstract] [Full Text]  
  • Mokas, S., Mills, J. R., Garreau, C., Fournier, M.-J., Robert, F., Arya, P., Kaufman, R. J., Pelletier, J., Mazroui, R. (2009). Uncoupling Stress Granule Assembly and Translation Initiation Inhibition. Mol. Biol. Cell 20: 2673-2683 [Abstract] [Full Text]  
  • Thomas, M. G., Tosar, L. J. M., Desbats, M. A., Leishman, C. C., Boccaccio, G. L. (2009). Mammalian Staufen 1 is recruited to stress granules and impairs their assembly. J. Cell Sci. 122: 563-573 [Abstract] [Full Text]  
  • Ward, A. M., Rekosh, D., Hammarskjold, M.-L. (2009). Trafficking through the Rev/RRE Pathway Is Essential for Efficient Inhibition of Human Immunodeficiency Virus Type 1 by an Antisense RNA Derived from the Envelope Gene. J. Virol. 83: 940-952 [Abstract] [Full Text]  
  • Ramirez-Valle, F., Braunstein, S., Zavadil, J., Formenti, S. C., Schneider, R. J. (2008). eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy. JCB 181: 293-307 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»