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Molecular and Cellular Biology, October 2007, p. 6686-6705, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00876-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Assembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae{triangledown} ,{dagger}

Koji Kasahara,1 Kazushige Ohtsuki,1 Sewon Ki,1 Kayo Aoyama,1 Hiroyuki Takahashi,1 Takehiko Kobayashi,2 Katsuhiko Shirahige,3 and Tetsuro Kokubo1*

Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama 230-0045, Japan,1 Division of Cytogenetics, National Institute of Genetics and SOKENDAI, Yata, Mishima 411-8540, Japan,2 Center for Biological Resources and Informatics, Division of Gene Research, and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8503, Japan3

Received 17 May 2007/ Returned for modification 12 June 2007/ Accepted 17 July 2007

HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1 dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner, whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence.

* Corresponding author. Mailing address: Division of Molecular and Cellular Biology, Science of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan. Phone: 45 508 7237. Fax: 45 508 7369. E-mail: kokubo{at}tsurumi.yokohama-cu.ac.jp

{triangledown} Published ahead of print on 23 July 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, October 2007, p. 6686-6705, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00876-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Merz, K., Hondele, M., Goetze, H., Gmelch, K., Stoeckl, U., Griesenbeck, J. (2008). Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. Genes Dev. 22: 1190-1204 [Abstract] [Full Text]  
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