This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eskeland, R.
Right arrow Articles by Imhof, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eskeland, R.
Right arrow Articles by Imhof, A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2007, p. 453-465, Vol. 27, No. 2
0270-7306/07/$08.00+0     doi:10.1128/MCB.01576-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

HP1 Binding to Chromatin Methylated at H3K9 Is Enhanced by Auxiliary Factors{triangledown}

Ragnhild Eskeland, Anton Eberharter, and Axel Imhof*

Adolf-Butenandt Institut, University of Munich, Schillerstr. 44, 80336 Muenchen, Germany

Received 23 August 2006/ Returned for modification 2 October 2006/ Accepted 1 November 2006

A large portion of the eukaryotic genome is packaged into transcriptionally silent heterochromatin. Several factors that play important roles during the establishment and maintenance of this condensed form have been identified. Methylation of lysine 9 within histone H3 and the subsequent binding of the chromodomain protein heterochromatin protein 1 (HP1) are thought to initiate heterochromatin formation in vivo and to propagate a heterochromatic state lasting through several cell divisions. For the present study we analyzed the binding of HP1 to methylated chromatin in a fully reconstituted system. In contrast to its strong binding to methylated peptides, HP1 binds only weakly to methylated chromatin. However, the addition of recombinant SU(VAR) protein, such as ACF1 or SU(VAR)3-9, facilitates HP1 binding to chromatin methylated at lysine 9 within the H3 N terminus (H3K9). We propose that HP1 has multiple target sites that contribute to its recognition of chromatin, only one of them being methylated at H3K9. These findings have implications for the mechanisms of recognition of specific chromatin modifications in vivo.

* Corresponding author. Mailing address: Histone Modifications Group, Adolf-Butenandt Institut, University of Munich, Schillerstr. 44, 80336 Muenchen, Germany. Phone: 49 89 218075420. Fax: 49 89 218075440. E-mail: Imhof{at}lmu.de.

{triangledown} Published ahead of print on 13 November 2006.

Molecular and Cellular Biology, January 2007, p. 453-465, Vol. 27, No. 2
0270-7306/07/$08.00+0     doi:10.1128/MCB.01576-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • LeRoy, G., Weston, J. T., Zee, B. M., Young, N. L., Plazas-Mayorca, M. D., Garcia, B. A. (2009). Heterochromatin Protein 1 Is Extensively Decorated with Histone Code-like Post-translational Modifications. Mol. Cell. Proteomics 8: 2432-2442 [Abstract] [Full Text]  
  • Chen, X., El Gazzar, M., Yoza, B. K., McCall, C. E. (2009). The NF-{kappa}B Factor RelB and Histone H3 Lysine Methyltransferase G9a Directly Interact to Generate Epigenetic Silencing in Endotoxin Tolerance. J. Biol. Chem. 284: 27857-27865 [Abstract] [Full Text]  
  • Luijsterburg, M. S., Dinant, C., Lans, H., Stap, J., Wiernasz, E., Lagerwerf, S., Warmerdam, D. O., Lindh, M., Brink, M. C., Dobrucki, J. W., Aten, J. A., Fousteri, M. I., Jansen, G., Dantuma, N. P., Vermeulen, W., Mullenders, L. H.F., Houtsmuller, A. B., Verschure, P. J., van Driel, R. (2009). Heterochromatin protein 1 is recruited to various types of DNA damage. JCB 185: 577-586 [Abstract] [Full Text]  
  • Scharf, A. N. D., Meier, K., Seitz, V., Kremmer, E., Brehm, A., Imhof, A. (2009). Monomethylation of Lysine 20 on Histone H4 Facilitates Chromatin Maturation. Mol. Cell. Biol. 29: 57-67 [Abstract] [Full Text]  
  • Gazzar, M. E., Yoza, B. K., Chen, X., Hu, J., Hawkins, G. A., McCall, C. E. (2008). G9a and HP1 Couple Histone and DNA Methylation to TNF{alpha} Transcription Silencing during Endotoxin Tolerance. J. Biol. Chem. 283: 32198-32208 [Abstract] [Full Text]  
  • Quenet, D., Gasser, V., Fouillen, L., Cammas, F., Sanglier-Cianferani, S., Losson, R., Dantzer, F. (2008). The histone subcode: poly(ADP-ribose) polymerase-1 (Parp-1) and Parp-2 control cell differentiation by regulating the transcriptional intermediary factor TIF1{beta} and the heterochromatin protein HP1{alpha}. FASEB J. 22: 3853-3865 [Abstract] [Full Text]  
  • Koh, A. S., Kuo, A. J., Park, S. Y., Cheung, P., Abramson, J., Bua, D., Carney, D., Shoelson, S. E., Gozani, O., Kingston, R. E., Benoist, C., Mathis, D. (2008). Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity. Proc. Natl. Acad. Sci. USA 105: 15878-15883 [Abstract] [Full Text]  
  • Honda, S., Selker, E. U. (2008). Direct Interaction between DNA Methyltransferase DIM-2 and HP1 Is Required for DNA Methylation in Neurospora crassa. Mol. Cell. Biol. 28: 6044-6055 [Abstract] [Full Text]  
  • Zhou, J., Wang, Q., Chen, L.-L., Carmichael, G. G. (2008). On the mechanism of induction of heterochromatin by the RNA-binding protein vigilin. RNA 14: 1773-1781 [Abstract] [Full Text]  
  • Schwendemann, A., Matkovic, T., Linke, C., Klebes, A., Hofmann, A., Korge, G. (2008). Hip, an HP1-interacting protein, is a haplo- and triplo-suppressor of position effect variegation. Proc. Natl. Acad. Sci. USA 105: 204-209 [Abstract] [Full Text]  
  • Dialynas, G. K., Terjung, S., Brown, J. P., Aucott, R. L., Baron-Luhr, B., Singh, P. B., Georgatos, S. D. (2007). Plasticity of HP1 proteins in mammalian cells. J. Cell Sci. 120: 3415-3424 [Abstract] [Full Text]  
  • Tzeng, T.-Y., Lee, C.-H., Chan, L.-W., Shen, C.-K. J. (2007). Epigenetic regulation of the Drosophila chromosome 4 by the histone H3K9 methyltransferase dSETDB1. Proc. Natl. Acad. Sci. USA 104: 12691-12696 [Abstract] [Full Text]  
  • Smith, E., Shilatifard, A. (2007). The A, B, Gs of silencing. Genes Dev. 21: 1141-1144 [Full Text]  
  • Smallwood, A., Esteve, P.-O., Pradhan, S., Carey, M. (2007). Functional cooperation between HP1 and DNMT1 mediates gene silencing. Genes Dev. 21: 1169-1178 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»