Next Article 
Molecular and Cellular Biology, October 2007, p. 6987-6995, Vol. 27, No. 20
0270-7306/07/$08.00+0 doi:10.1128/MCB.00717-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
The Swi/Snf Complex Is Important for Histone Eviction during Transcriptional Activation and RNA Polymerase II Elongation In Vivo
,
Marc A. Schwabish and
Kevin Struhl*
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
Received 24 April 2007/
Returned for modification 1 June 2007/
Accepted 9 August 2007
The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.
* Corresponding author. Mailing address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115. Phone: (617) 432-2104. Fax: (617) 432-2529. E-mail:
kevin{at}hms.harvard.edu
Published ahead of print on 20 August 2007.
Supplemental material for this article may be found at http://mcb.asm.org/.
Molecular and Cellular Biology, October 2007, p. 6987-6995, Vol. 27, No. 20
0270-7306/07/$08.00+0 doi:10.1128/MCB.00717-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Ginsburg, D. S., Govind, C. K., Hinnebusch, A. G.
(2009). NuA4 Lysine Acetyltransferase Esa1 Is Targeted to Coding Regions and Stimulates Transcription Elongation with Gcn5. Mol. Cell. Biol.
29: 6473-6487
[Abstract]
[Full Text]
-
Selth, L. A., Lorch, Y., Ocampo-Hafalla, M. T., Mitter, R., Shales, M., Krogan, N. J., Kornberg, R. D., Svejstrup, J. Q.
(2009). An Rtt109-Independent Role for Vps75 in Transcription-Associated Nucleosome Dynamics. Mol. Cell. Biol.
29: 4220-4234
[Abstract]
[Full Text]
-
Gkikopoulos, T., Havas, K. M., Dewar, H., Owen-Hughes, T.
(2009). SWI/SNF and Asf1p Cooperate To Displace Histones during Induction of the Saccharomyces cerevisiae HO Promoter. Mol. Cell. Biol.
29: 4057-4066
[Abstract]
[Full Text]
-
Mao, X., Nie, X., Cao, F., Chen, J.
(2009). Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae. Acta Biochim Biophys Sin
41: 594-602
[Abstract]
[Full Text]
-
Cliffe, A. R., Knipe, D. M.
(2008). Herpes Simplex Virus ICP0 Promotes both Histone Removal and Acetylation on Viral DNA during Lytic Infection. J. Virol.
82: 12030-12038
[Abstract]
[Full Text]