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Molecular and Cellular Biology, October 2007, p. 6987-6995, Vol. 27, No. 20
0270-7306/07/$08.00+0     doi:10.1128/MCB.00717-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Swi/Snf Complex Is Important for Histone Eviction during Transcriptional Activation and RNA Polymerase II Elongation In Vivo{triangledown} ,{dagger}

Marc A. Schwabish and Kevin Struhl*

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115

Received 24 April 2007/ Returned for modification 1 June 2007/ Accepted 9 August 2007

The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.

* Corresponding author. Mailing address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115. Phone: (617) 432-2104. Fax: (617) 432-2529. E-mail: kevin{at}hms.harvard.edu

{triangledown} Published ahead of print on 20 August 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, October 2007, p. 6987-6995, Vol. 27, No. 20
0270-7306/07/$08.00+0     doi:10.1128/MCB.00717-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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