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Molecular and Cellular Biology, October 2007, p. 7176-7187, Vol. 27, No. 20
0270-7306/07/$08.00+0     doi:10.1128/MCB.00696-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Regulation of the Extrinsic Apoptotic Pathway by the Extracellular Matrix Glycoprotein EMILIN2 ^,

Maurizio Mongiat,^1* Giovanni Ligresti,¹ Stefano Marastoni,¹ Erica Lorenzon,¹ Roberto Doliana,¹ and Alfonso Colombatti^1,2,3*

Department of Molecular Oncology and Translational Research, Experimental Division 2, National Cancer Institute CRO-IRCCS, Aviano, Italy,¹ Department of Sciences and Biomedical Technology, University of Udine, Udine, Italy,² MATI Center of Excellence, University of Udine, Udine, Italy³

Received 20 April 2007/ Returned for modification 11 May 2007/ Accepted 31 July 2007

Elastin microfibril interface-located proteins (EMILINs) constitute a family of extracellular matrix (ECM) glycoproteins characterized by the presence of an EMI domain at the N terminus and a gC1q domain at the C terminus. EMILIN1, the archetype molecule of the family, is involved in elastogenesis and hypertension etiology, whereas the function of EMILIN2 has not been resolved. Here, we provide evidence that the expression of EMILIN2 triggers the apoptosis of different cell lines. Cell death depends on the activation of the extrinsic apoptotic pathway following EMILIN2 binding to the TRAIL receptors DR4 and, to a lesser extent, DR5. Binding is followed by receptor clustering, colocalization with lipid rafts, death-inducing signaling complex assembly, and caspase activation. The direct activation of death receptors by an ECM molecule that mimics the activity of the known death receptor ligands is novel. The knockdown of EMILIN2 increases transformed cell survival, and overexpression impairs clonogenicity in soft agar and three-dimensional growth in natural matrices due to massive apoptosis. These data demonstrate an unexpected direct and functional interaction of an ECM constituent with death receptors and discloses an additional mechanism by which ECM cues can negatively affect cell survival.

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* Corresponding author. Mailing address: Department of Molecular Oncology and Translational Research, Experimental Division 2, CRO-IRCCS, via Pedemontana Occidentale 12, 33081 Aviano (PN), Italy. Phone for Alfonso Colombatti: 39 0434 659 301. Fax: 39 0434 659 428. E-mail: acolombatti{at}cro.it. Phone for Maurizio Mongiat: 39 0434 659 760. Fax: 39 0434 659 428. E-mail: mmongiat{at}cro.it

Published ahead of print on 13 August 2007.

Supplemental material for this article may be found at http://mcb.asm.org/.

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Molecular and Cellular Biology, October 2007, p. 7176-7187, Vol. 27, No. 20
0270-7306/07/$08.00+0     doi:10.1128/MCB.00696-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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