This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ciesla, M.
Right arrow Articles by Boguta, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ciesla, M.
Right arrow Articles by Boguta, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2007, p. 7693-7702, Vol. 27, No. 21
0270-7306/07/$08.00+0     doi:10.1128/MCB.01051-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Maf1 Is Involved in Coupling Carbon Metabolism to RNA Polymerase III Transcription{triangledown} ,{dagger}

Malgorzata Ciesla,1 Joanna Towpik,1 Damian Graczyk,1 Danuta Oficjalska-Pham,1,2,{ddagger} Olivier Harismendy,2,§ Audrey Suleau,2 Karol Balicki,1 Christine Conesa,2 Olivier Lefebvre,2 and Magdalena Boguta1*

Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland,1 CEA, iBiTecS, Gif Sur Yvette F-91191, France2

Received 14 June 2007/ Returned for modification 4 July 2007/ Accepted 23 August 2007

RNA polymerase III (Pol III) produces essential components of the biosynthetic machinery, and therefore its activity is tightly coupled with cell growth and metabolism. In the yeast Saccharomyces cerevisiae, Maf1 is the only known global and direct Pol III transcription repressor which mediates numerous stress signals. Here we demonstrate that transcription regulation by Maf1 is not limited to stress but is important for the switch between fermentation and respiration. Under respiratory conditions, Maf1 is activated by dephosphorylation and imported into the nucleus. The transition from a nonfermentable carbon source to that of glucose induces Maf1 phosphorylation and its relocation to the cytoplasm. The absence of Maf1-mediated control of tRNA synthesis impairs cell viability in nonfermentable carbon sources. The respiratory phenotype of maf1-{Delta} allowed genetic suppression studies to dissect the mechanism of Maf1 action on the Pol III transcription apparatus. Moreover, in cells grown in a nonfermentable carbon source, Maf1 regulates the levels of different tRNAs to various extents. The differences in regulation may contribute to the physiological role of Maf1.

* Corresponding author. Mailing address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland. Phone: 4822 592 1312. Fax: 4822 658 4636. E-mail: magda{at}ibb.waw.pl

{triangledown} Published ahead of print on 4 September 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Present address: Unit of Epigenetic Regulation, Avenir INSERM-FRE2850 CNRS, Fernbach Bldg., Institut Pasteur, 25 rue du Dr-Roux, 75724 Paris CEDEX 15, France.

§ Present address: Department of Neurobiology, Scripps Research Institute, 10550 North Torrey Pines Road, San Diego, CA 92037.

Molecular and Cellular Biology, November 2007, p. 7693-7702, Vol. 27, No. 21
0270-7306/07/$08.00+0     doi:10.1128/MCB.01051-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boisnard, S., Lagniel, G., Garmendia-Torres, C., Molin, M., Boy-Marcotte, E., Jacquet, M., Toledano, M. B., Labarre, J., Chedin, S. (2009). H2O2 Activates the Nuclear Localization of Msn2 and Maf1 through Thioredoxins in Saccharomyces cerevisiae. Eukaryot Cell 8: 1429-1438 [Abstract] [Full Text]  
  • Lee, J., Moir, R. D., Willis, I. M. (2009). Regulation of RNA Polymerase III Transcription Involves SCH9-dependent and SCH9-independent Branches of the Target of Rapamycin (TOR) Pathway. J. Biol. Chem. 284: 12604-12608 [Abstract] [Full Text]  
  • Soragni, E., Kassavetis, G. A. (2008). Absolute Gene Occupancies by RNA Polymerase III, TFIIIB, and TFIIIC in Saccharomyces cerevisiae. J. Biol. Chem. 283: 26568-26576 [Abstract] [Full Text]  
  • Ghavi-Helm, Y., Michaut, M., Acker, J., Aude, J.-C., Thuriaux, P., Werner, M., Soutourina, J. (2008). Genome-wide location analysis reveals a role of TFIIS in RNA polymerase III transcription. Genes Dev. 22: 1934-1947 [Abstract] [Full Text]  
  • Towpik, J., Graczyk, D., Gajda, A., Lefebvre, O., Boguta, M. (2008). Derepression of RNA Polymerase III Transcription by Phosphorylation and Nuclear Export of Its Negative Regulator, Maf1. J. Biol. Chem. 283: 17168-17174 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»